103198

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3036168..3036670 Replicon chromosome
Accession NZ_CP028735
Organism Escherichia coli strain A41

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag AOY94_RS14575 Protein ID WP_000767828.1
Coordinates 3036168..3036422 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AOY94_RS14580 Protein ID WP_001259255.1
Coordinates 3036419..3036670 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY94_RS14545 (3031183) 3031183..3031410 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
AOY94_RS14550 (3031424) 3031424..3032482 - 1059 WP_000492344.1 thiosulfate utilization transporter TsuA/YeeE -
AOY94_RS14555 (3032661) 3032661..3034019 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AOY94_RS14560 (3034009) 3034009..3034071 - 63 WP_010723108.1 membrane protein YoeI -
AOY94_RS14565 (3034286) 3034286..3035215 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
AOY94_RS14570 (3035261) 3035261..3036085 - 825 WP_000754758.1 SDR family oxidoreductase -
AOY94_RS14575 (3036168) 3036168..3036422 - 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AOY94_RS14580 (3036419) 3036419..3036670 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AOY94_RS14585 (3036953) 3036953..3037003 + 51 WP_001364200.1 his operon leader peptide -
AOY94_RS14590 (3037149) 3037149..3038048 + 900 WP_000131783.1 ATP phosphoribosyltransferase -
AOY94_RS14595 (3038054) 3038054..3039358 + 1305 WP_096932751.1 histidinol dehydrogenase -
AOY94_RS14600 (3039355) 3039355..3040425 + 1071 WP_001564244.1 histidinol-phosphate transaminase -
AOY94_RS14605 (3040425) 3040425..3041492 + 1068 WP_000080130.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T103198 WP_000767828.1 NZ_CP028735:c3036422-3036168 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T103198 NZ_CP028735:c3036422-3036168 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT103198 WP_001259255.1 NZ_CP028735:c3036670-3036419 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT103198 NZ_CP028735:c3036670-3036419 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References