Detailed information of TA system TA103185

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2332316..2332687
• Replicon: chromosome
• Accession: NZ_CP028735
• Organism: Escherichia coli strain A41

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A3L9BYF7
• Locus tag: AOY94_RS11115
• Protein ID: WP_072668032.1
• Coordinates: 2332493..2332687 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2332316..2332494 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY94_RS24950 (2328068)	2328068..2328241	+	174	WP_001296046.1	protein YnaL	-
AOY94_RS11090 (2328271)	2328271..2329644	+	1374	WP_000123745.1	ATP-dependent RNA helicase DbpA	-
AOY94_RS11095 (2329773)	2329773..2330708	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
AOY94_RS11100 (2330760)	2330760..2331995	-	1236	WP_240764565.1	site-specific integrase	-
AOY94_RS11105 (2331997)	2331997..2332212	-	216	WP_000079604.1	excisionase XisR	-
- (2332316)	2332316..2332494	+	179	NuclAT_0	-	Antitoxin
- (2332316)	2332316..2332494	+	179	NuclAT_0	-	Antitoxin
- (2332316)	2332316..2332494	+	179	NuclAT_0	-	Antitoxin
- (2332316)	2332316..2332494	+	179	NuclAT_0	-	Antitoxin
AOY94_RS11110 (2332312)	2332312..2332500	-	189	WP_001302840.1	DUF1187 family protein	-
AOY94_RS11115 (2332493)	2332493..2332687	-	195	WP_072668032.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
AOY94_RS11120 (2332744)	2332744..2333553	-	810	WP_000166317.1	recombination protein RecT	-
AOY94_RS11125 (2333546)	2333546..2336197	-	2652	WP_240764566.1	exodeoxyribonuclease VIII	-
AOY94_RS11130 (2336296)	2336296..2336570	-	275	Protein_2167	hypothetical protein	-
AOY94_RS11135 (2336644)	2336644..2336814	-	171	WP_240764567.1	Rtr1/RPAP2 family protein phosphatase	-
AOY94_RS11140 (2336814)	2336814..2337035	-	222	WP_000560218.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2330760..2375751	44991

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.93 Da
• Isoelectric Point: 9.2886

>T103185 WP_072668032.1 NZ_CP028735:c2332687-2332493 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSKKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T103185 NZ_CP028735:c2332687-2332493 [Escherichia coli]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTAAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT103185 NZ_CP028735:2332316-2332494 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A3L9BYF7

Antitoxin