103182

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1178921..1179462 Replicon chromosome
Accession NZ_CP028735
Organism Escherichia coli strain A41

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag AOY94_RS05575 Protein ID WP_000615976.1
Coordinates 1178921..1179199 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY94_RS05580 Protein ID WP_000729704.1
Coordinates 1179202..1179462 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY94_RS05560 (1176503) 1176503..1177081 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AOY94_RS05565 (1177287) 1177287..1178054 + 768 WP_000333380.1 class II glutamine amidotransferase -
AOY94_RS05570 (1178025) 1178025..1178765 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY94_RS05575 (1178921) 1178921..1179199 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY94_RS05580 (1179202) 1179202..1179462 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY94_RS05585 (1179648) 1179648..1180421 + 774 WP_000543899.1 C40 family peptidase -
AOY94_RS05590 (1180478) 1180478..1180834 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
AOY94_RS05595 (1180827) 1180827..1181105 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AOY94_RS05600 (1181210) 1181210..1183303 - 2094 WP_001140827.1 flagellar biosynthesis protein FlhA -
AOY94_RS05605 (1183287) 1183287..1184426 - 1140 WP_000785664.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T103182 WP_000615976.1 NZ_CP028735:c1179199-1178921 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103182 NZ_CP028735:c1179199-1178921 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT103182 WP_000729704.1 NZ_CP028735:c1179462-1179202 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103182 NZ_CP028735:c1179462-1179202 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6Z6

References