Detailed information of TA system TA103177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 152118..152720
• Replicon: chromosome
• Accession: NZ_CP028735
• Organism: Escherichia coli strain A41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY94_RS00740
• Protein ID: WP_000897305.1
• Coordinates: 152118..152429 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY94_RS00745
• Protein ID: WP_000356397.1
• Coordinates: 152430..152720 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY94_RS00710 (147148)	147148..147933	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AOY94_RS00715 (148032)	148032..148631	+	600	WP_001295269.1	glucose-1-phosphatase	-
AOY94_RS00720 (148625)	148625..149497	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY94_RS00725 (149494)	149494..149931	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY94_RS00730 (149976)	149976..150917	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY94_RS00735 (150981)	150981..151889	-	909	WP_001162704.1	alpha/beta hydrolase	-
AOY94_RS00740 (152118)	152118..152429	+	312	WP_000897305.1	hypothetical protein	Toxin
AOY94_RS00745 (152430)	152430..152720	+	291	WP_000356397.1	NadS family protein	Antitoxin
AOY94_RS00750 (153325)	153325..153543	+	219	WP_001295676.1	CopG family transcriptional regulator	-
AOY94_RS00755 (153762)	153762..154004	+	243	WP_001086388.1	protein YiiF	-
AOY94_RS00760 (154334)	154334..155263	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AOY94_RS00765 (155260)	155260..155895	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY94_RS00770 (155892)	155892..156794	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103177 WP_000897305.1 NZ_CP028735:152118-152429 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103177 NZ_CP028735:152118-152429 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103177 WP_000356397.1 NZ_CP028735:152430-152720 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103177 NZ_CP028735:152430-152720 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7