Detailed information of TA system TA103176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4575445..4576047
• Replicon: chromosome
• Accession: NZ_CP028734
• Organism: Escherichia coli strain A42

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3L4YH82
• Locus tag: AOY95_RS22035
• Protein ID: WP_000897303.1
• Coordinates: 4575736..4576047 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY95_RS22030
• Protein ID: WP_000356397.1
• Coordinates: 4575445..4575735 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY95_RS22000 (4570602)	4570602..4571531	+	930	WP_001523575.1	formate dehydrogenase accessory protein FdhE	-
AOY95_RS22005 (4571861)	4571861..4572103	-	243	WP_001523574.1	CopG family transcriptional regulator	-
AOY95_RS22010 (4572393)	4572393..4573241	+	849	WP_001038651.1	hypothetical protein	-
AOY95_RS22015 (4573317)	4573317..4574006	+	690	WP_224916703.1	hypothetical protein	-
AOY95_RS22020 (4574191)	4574191..4574409	-	219	WP_001251293.1	CopG family transcriptional regulator	-
AOY95_RS22025 (4574808)	4574808..4575086	-	279	WP_001296612.1	hypothetical protein	-
AOY95_RS22030 (4575445)	4575445..4575735	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY95_RS22035 (4575736)	4575736..4576047	-	312	WP_000897303.1	hypothetical protein	Toxin
AOY95_RS22040 (4576276)	4576276..4577184	+	909	WP_001385591.1	alpha/beta hydrolase	-
AOY95_RS22045 (4577248)	4577248..4578189	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY95_RS22050 (4578234)	4578234..4578671	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY95_RS22055 (4578668)	4578668..4579540	-	873	WP_096950750.1	virulence factor BrkB family protein	-
AOY95_RS22060 (4579534)	4579534..4580133	-	600	WP_001298594.1	glucose-1-phosphatase	-
AOY95_RS22065 (4580232)	4580232..4581017	-	786	WP_001523571.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12302.33 Da
• Isoelectric Point: 10.0233

>T103176 WP_000897303.1 NZ_CP028734:c4576047-4575736 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGRLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103176 NZ_CP028734:c4576047-4575736 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTAGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAGGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103176 WP_000356397.1 NZ_CP028734:c4575735-4575445 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103176 NZ_CP028734:c4575735-4575445 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A3L4YH82