Detailed information of TA system TA103171

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-rdlD/SymE(toxin)
• Location: 4032522..4032931
• Replicon: chromosome
• Accession: NZ_CP028734
• Organism: Escherichia coli strain A42

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A3L4RJH9
• Locus tag: AOY95_RS19565
• Protein ID: WP_001357525.1
• Coordinates: 4032593..4032931 (+)
• Length: 113 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	4032522..4032598 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY95_RS19545 (4028589)	4028589..4028792	+	204	WP_000467859.1	YbdD/YjiX family protein	-
AOY95_RS19550 (4028803)	4028803..4029759	+	957	WP_001295525.1	GTPase	-
AOY95_RS19555 (4030090)	4030090..4031940	+	1851	WP_000190966.1	nuclease-related domain-containing DEAD/DEAH box helicase	-
AOY95_RS19560 (4032169)	4032169..4032378	+	210	Protein_3837	DUF3387 domain-containing protein	-
- (4032522)	4032522..4032598	-	77	NuclAT_12	-	Antitoxin
- (4032522)	4032522..4032598	-	77	NuclAT_12	-	Antitoxin
- (4032522)	4032522..4032598	-	77	NuclAT_12	-	Antitoxin
- (4032522)	4032522..4032598	-	77	NuclAT_12	-	Antitoxin
- (4032522)	4032522..4032598	-	77	NuclAT_13	-	Antitoxin
- (4032522)	4032522..4032598	-	77	NuclAT_13	-	Antitoxin
- (4032522)	4032522..4032598	-	77	NuclAT_13	-	Antitoxin
- (4032522)	4032522..4032598	-	77	NuclAT_13	-	Antitoxin
AOY95_RS19565 (4032593)	4032593..4032931	+	339	WP_001357525.1	endoribonuclease SymE	Toxin
AOY95_RS19570 (4033019)	4033019..4033153	-	135	WP_230158887.1	hypothetical protein	-
AOY95_RS19575 (4033315)	4033315..4033629	+	315	Protein_3840	winged helix-turn-helix domain-containing protein	-
AOY95_RS19580 (4033621)	4033621..4034541	-	921	WP_001523888.1	Rpn family recombination-promoting nuclease/putative transposase	-
AOY95_RS19585 (4034726)	4034726..4036006	+	1281	WP_001523887.1	DUF445 domain-containing protein	-
AOY95_RS19590 (4036124)	4036124..4037275	+	1152	WP_001298064.1	double-cubane-cluster-containing anaerobic reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12314.03 Da
• Isoelectric Point: 7.8234

>T103171 WP_001357525.1 NZ_CP028734:4032593-4032931 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFTTGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQRQVQDFIGVISSKTPR

Nucleotide

• Length: 339 bp

>T103171 NZ_CP028734:4032593-4032931 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGCCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTACCA
CCGGCACGGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCACTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGATTTTATTGGGGTGATTAG
TAGCAAAACGCCACGCTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT103171 NZ_CP028734:c4032598-4032522 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A3L4RJH9

Antitoxin