Detailed information of TA system TA103169

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3637500..3638194
• Replicon: chromosome
• Accession: NZ_CP028734
• Organism: Escherichia coli strain A42

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: AOY95_RS17690
• Protein ID: WP_001263500.1
• Coordinates: 3637500..3637898 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AOY95_RS17695
• Protein ID: WP_000554758.1
• Coordinates: 3637901..3638194 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY95_RS17665 (3632866)	3632866..3633324	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
AOY95_RS17670 (3633585)	3633585..3635042	+	1458	WP_001293022.1	cytosol nonspecific dipeptidase	-
AOY95_RS17675 (3635098)	3635098..3635712	-	615	WP_000602123.1	peptide chain release factor H	-
AOY95_RS17680 (3635709)	3635709..3636848	-	1140	WP_001606885.1	RNA ligase RtcB family protein	-
AOY95_RS17685 (3637038)	3637038..3637490	-	453	WP_071589393.1	GNAT family N-acetyltransferase	-
AOY95_RS17690 (3637500)	3637500..3637898	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AOY95_RS17695 (3637901)	3637901..3638194	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AOY95_RS17700 (3638246)	3638246..3639301	-	1056	WP_001226168.1	DNA polymerase IV	-
AOY95_RS17705 (3639372)	3639372..3640295	-	924	WP_218068696.1	putative lateral flagellar export/assembly protein LafU	-
AOY95_RS17710 (3640298)	3640298..3641161	-	864	WP_001169514.1	flagellar motor stator protein MotA	-
AOY95_RS17715 (3641174)	3641174..3641890	-	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
AOY95_RS17720 (3641910)	3641910..3642377	-	468	WP_000725265.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T103169 WP_001263500.1 NZ_CP028734:c3637898-3637500 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T103169 NZ_CP028734:c3637898-3637500 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT103169 WP_000554758.1 NZ_CP028734:c3638194-3637901 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT103169 NZ_CP028734:c3638194-3637901 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3