Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2464538..2465100 Replicon chromosome
Accession NZ_CP028734
Organism Escherichia coli strain A42

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag AOY95_RS11940 Protein ID WP_000605675.1
Coordinates 2464538..2464816 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AOY95_RS11945 Protein ID WP_000781370.1
Coordinates 2464816..2465100 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY95_RS11915 (2460125) 2460125..2460556 - 432 WP_000152305.1 peroxiredoxin OsmC -
AOY95_RS11920 (2460901) 2460901..2461116 + 216 WP_000495766.1 biofilm-dependent modulation protein -
AOY95_RS11925 (2461218) 2461218..2461355 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AOY95_RS11930 (2461511) 2461511..2463208 + 1698 WP_000433462.1 malate dehydrogenase -
AOY95_RS11935 (2463342) 2463342..2464352 + 1011 WP_000642417.1 alcohol dehydrogenase AdhP -
AOY95_RS11940 (2464538) 2464538..2464816 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AOY95_RS11945 (2464816) 2464816..2465100 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
AOY95_RS11950 (2465328) 2465328..2465981 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
AOY95_RS11955 (2465974) 2465974..2466858 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
AOY95_RS11960 (2466871) 2466871..2469918 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T103166 WP_000605675.1 NZ_CP028734:2464538-2464816 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T103166 NZ_CP028734:2464538-2464816 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT103166 WP_000781370.1 NZ_CP028734:2464816-2465100 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT103166 NZ_CP028734:2464816-2465100 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References