Detailed information of TA system TA103146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4659224..4659826
• Replicon: chromosome
• Accession: NZ_CP028733
• Organism: Escherichia coli strain H2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AOY59_RS22635
• Protein ID: WP_000897305.1
• Coordinates: 4659515..4659826 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOY59_RS22630
• Protein ID: WP_000356397.1
• Coordinates: 4659224..4659514 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOY59_RS22605 (4655150)	4655150..4656052	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AOY59_RS22610 (4656049)	4656049..4656684	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AOY59_RS22615 (4656681)	4656681..4657610	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AOY59_RS22620 (4657940)	4657940..4658182	-	243	WP_001086388.1	protein YiiF	-
AOY59_RS22625 (4658401)	4658401..4658619	-	219	WP_001295676.1	CopG family transcriptional regulator	-
AOY59_RS22630 (4659224)	4659224..4659514	-	291	WP_000356397.1	NadS family protein	Antitoxin
AOY59_RS22635 (4659515)	4659515..4659826	-	312	WP_000897305.1	hypothetical protein	Toxin
AOY59_RS22640 (4660055)	4660055..4660963	+	909	WP_001162704.1	alpha/beta hydrolase	-
AOY59_RS22645 (4661027)	4661027..4661968	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AOY59_RS22650 (4662013)	4662013..4662450	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
AOY59_RS22655 (4662447)	4662447..4663319	-	873	WP_000920762.1	virulence factor BrkB family protein	-
AOY59_RS22660 (4663313)	4663313..4663912	-	600	WP_001315111.1	glucose-1-phosphatase	-
AOY59_RS22665 (4664011)	4664011..4664796	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T103146 WP_000897305.1 NZ_CP028733:c4659826-4659515 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T103146 NZ_CP028733:c4659826-4659515 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT103146 WP_000356397.1 NZ_CP028733:c4659514-4659224 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT103146 NZ_CP028733:c4659514-4659224 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7