Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3810407..3810948 Replicon chromosome
Accession NZ_CP028733
Organism Escherichia coli strain H2

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag AOY59_RS18605 Protein ID WP_000615976.1
Coordinates 3810670..3810948 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag AOY59_RS18600 Protein ID WP_000729704.1
Coordinates 3810407..3810667 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY59_RS18575 (3805443) 3805443..3806582 + 1140 WP_096948622.1 flagellar type III secretion system protein FlhB -
AOY59_RS18580 (3806566) 3806566..3808659 + 2094 WP_001597626.1 flagellar biosynthesis protein FlhA -
AOY59_RS18585 (3808764) 3808764..3809042 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
AOY59_RS18590 (3809035) 3809035..3809391 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
AOY59_RS18595 (3809448) 3809448..3810221 - 774 WP_000543895.1 C40 family peptidase -
AOY59_RS18600 (3810407) 3810407..3810667 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY59_RS18605 (3810670) 3810670..3810948 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY59_RS18610 (3811104) 3811104..3811844 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY59_RS18615 (3811815) 3811815..3812582 - 768 WP_000333380.1 class II glutamine amidotransferase -
AOY59_RS18620 (3812788) 3812788..3813366 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR 3809035..3827660 18625


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T103144 WP_000615976.1 NZ_CP028733:3810670-3810948 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103144 NZ_CP028733:3810670-3810948 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT103144 WP_000729704.1 NZ_CP028733:3810407-3810667 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103144 NZ_CP028733:3810407-3810667 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References