Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3004041..3004543 Replicon chromosome
Accession NZ_CP028732
Organism Escherichia coli strain H3

Toxin (Protein)


Gene name yoeB Uniprot ID D3GZ85
Locus tag AOY60_RS14660 Protein ID WP_000767820.1
Coordinates 3004041..3004295 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AOY60_RS14665 Protein ID WP_001259255.1
Coordinates 3004292..3004543 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY60_RS14630 (2999056) 2999056..2999283 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
AOY60_RS14635 (2999297) 2999297..3000355 - 1059 WP_201473011.1 thiosulfate utilization transporter TsuA/YeeE -
AOY60_RS14640 (3000534) 3000534..3001892 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AOY60_RS14645 (3001882) 3001882..3001944 - 63 WP_010723108.1 membrane protein YoeI -
AOY60_RS14650 (3002159) 3002159..3003088 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
AOY60_RS14655 (3003134) 3003134..3003958 - 825 WP_000754756.1 SDR family oxidoreductase -
AOY60_RS14660 (3004041) 3004041..3004295 - 255 WP_000767820.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AOY60_RS14665 (3004292) 3004292..3004543 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AOY60_RS14670 (3004827) 3004827..3004877 + 51 WP_001364200.1 his operon leader peptide -
AOY60_RS14675 (3005023) 3005023..3005922 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
AOY60_RS14680 (3005928) 3005928..3007232 + 1305 WP_039025710.1 histidinol dehydrogenase -
AOY60_RS14685 (3007229) 3007229..3008299 + 1071 WP_000108952.1 histidinol-phosphate transaminase -
AOY60_RS14690 (3008299) 3008299..3009366 + 1068 WP_086173928.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10182.64 Da        Isoelectric Point: 8.0353

>T103119 WP_000767820.1 NZ_CP028732:c3004295-3004041 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKIIELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T103119 NZ_CP028732:c3004295-3004041 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT103119 WP_001259255.1 NZ_CP028732:c3004543-3004292 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT103119 NZ_CP028732:c3004543-3004292 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGCCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D3GZ85

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References