103104

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1020042..1020583 Replicon chromosome
Accession NZ_CP028732
Organism Escherichia coli strain H3

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag AOY60_RS04840 Protein ID WP_000615983.1
Coordinates 1020042..1020320 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag AOY60_RS04845 Protein ID WP_000729703.1
Coordinates 1020323..1020583 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOY60_RS04825 (1017624) 1017624..1018202 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
AOY60_RS04830 (1018408) 1018408..1019175 + 768 WP_000333380.1 class II glutamine amidotransferase -
AOY60_RS04835 (1019146) 1019146..1019886 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
AOY60_RS04840 (1020042) 1020042..1020320 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
AOY60_RS04845 (1020323) 1020323..1020583 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
AOY60_RS04850 (1020793) 1020793..1021542 + 750 WP_000056849.1 C40 family peptidase -
AOY60_RS04855 (1021718) 1021718..1022215 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
AOY60_RS04860 (1022439) 1022439..1024151 - 1713 Protein_935 flagellar biosynthesis protein FlhA -
AOY60_RS04865 (1024123) 1024123..1024908 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - hcp1/tssD1 / tssA / rhs/PAAR / gmhA/lpcA 999352..1038864 39512


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T103104 WP_000615983.1 NZ_CP028732:c1020320-1020042 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T103104 NZ_CP028732:c1020320-1020042 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT103104 WP_000729703.1 NZ_CP028732:c1020583-1020323 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT103104 NZ_CP028732:c1020583-1020323 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
AlphaFold DB A0A5F1F716

References