Detailed information of TA system TA103073

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 1306693..1307033
• Replicon: chromosome
• Accession: NZ_CP028724
• Organism: Enterococcus faecalis strain CVM N60443F

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: A0A4U4MH53
• Locus tag: C7I31_RS06415
• Protein ID: WP_073340330.1
• Coordinates: 1306693..1306845 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	1306778..1307033 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7I31_RS06390	1301883..1302098	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
C7I31_RS06395	1302237..1303229	+	993	WP_010778026.1	biotin--[acetyl-CoA-carboxylase] ligase	-
C7I31_RS06400	1303493..1304131	-	639	WP_002375448.1	lytic polysaccharide monooxygenase	-
C7I31_RS06410	1304816..1306432	+	1617	WP_131639190.1	phosphatase PAP2/LCP family protein	-
C7I31_RS06415	1306693..1306845	+	153	WP_073340330.1	putative holin-like toxin	Toxin
-	1306778..1307033	-	256	NuclAT_3	-	Antitoxin
C7I31_RS06420	1307035..1310814	-	3780	WP_131639191.1	WxL domain-containing protein	-
-	1311155..1311312	-	158	NuclAT_5	-	-
-	1311165..1311298	-	134	NuclAT_11	-	-
C7I31_RS06425	1311409..1311552	+	144	WP_002396786.1	putative holin-like toxin	-
-	1311484..1311697	-	214	NuclAT_10	-	-
-	1311740..1311789	+	50	NuclAT_14	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (17-50)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5665.96 Da
• Isoelectric Point: 11.0942

>T103073 WP_073340330.1 NZ_CP028724:1306693-1306845 [Enterococcus faecalis]
VVRMSVLPKFTERRGLLSAYETIQTILGFGMFTIALIALIVKLLKNDKKK

Nucleotide

• Length: 153 bp

>T103073 NZ_CP028724:1306693-1306845 [Enterococcus faecalis]
GTGGTGCGTATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTTTTGTCAGCATATGAGACAATTCAGACGATTCT
AGGTTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Nucleotide

• Length: 256 bp

>AT103073 NZ_CP028724:c1307033-1306778 [Enterococcus faecalis]
ATAAAAATGCCCGAGCCTTGAAATAGGAGGCTCAGGCATTTTTTATTGATGGTCTTTTTAGGGTTGTTGTGCTATAATGA
TAAAGAAAAGAGAGATATGCTTCCAACATATCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCGTGTTAATTATTTAAA
AATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCAATCAAAGCA
ATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A4U4MH53

Antitoxin