Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2949278..2949780 Replicon chromosome
Accession NZ_CP028703
Organism Escherichia coli strain ME8067

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DBB47_RS15490 Protein ID WP_000767829.1
Coordinates 2949278..2949532 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DBB47_RS15495 Protein ID WP_001259255.1
Coordinates 2949529..2949780 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBB47_RS15460 2944293..2944520 - 228 WP_000234896.1 sulfurtransferase TusA -
DBB47_RS15465 2944534..2945592 - 1059 WP_000492339.1 transport protein YeeE -
DBB47_RS15470 2945771..2947129 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DBB47_RS15475 2947119..2947181 - 63 WP_010723108.1 membrane protein YoeI -
DBB47_RS15480 2947396..2948325 - 930 WP_000803351.1 LysR family transcriptional regulator -
DBB47_RS15485 2948371..2949195 - 825 WP_000754737.1 SDR family oxidoreductase -
DBB47_RS15490 2949278..2949532 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DBB47_RS15495 2949529..2949780 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DBB47_RS15500 2950063..2950113 + 51 WP_001364200.1 his operon leader peptide -
DBB47_RS15510 2950259..2951158 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DBB47_RS15515 2951164..2952468 + 1305 WP_000009594.1 histidinol dehydrogenase -
DBB47_RS15520 2952465..2953535 + 1071 WP_108118748.1 histidinol-phosphate transaminase -
DBB47_RS15525 2953535..2954602 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T103012 WP_000767829.1 NZ_CP028703:c2949532-2949278 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T103012 NZ_CP028703:c2949532-2949278 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT103012 WP_001259255.1 NZ_CP028703:c2949780-2949529 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT103012 NZ_CP028703:c2949780-2949529 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References