Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/Xre-RelE |
| Location | 2201584..2202587 | Replicon | chromosome |
| Accession | NC_000962 | ||
| Organism | Mycobacterium tuberculosis H37Rv | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | P9WJA5 |
| Locus tag | Rv1955 | Protein ID | NP_216471.2 |
| Coordinates | 2201719..2202096 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | P9WJA7 |
| Locus tag | Rv1956 | Protein ID | NP_216472.1 |
| Coordinates | 2202138..2202587 (+) | Length | 150 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Rv1946c | 2197508..2197960 | - | 453 | NP_216462.1 | lipoprotein | - |
| Rv1947 | 2198024..2198425 | + | 402 | NP_216463.1 | hypothetical protein | - |
| Rv1948c | 2198714..2199064 | - | 351 | NP_216464.1 | hypothetical protein | - |
| Rv1950c | 2199998..2200189 | - | 192 | NP_216466.1 | hypothetical protein | - |
| Rv1951c | 2200190..2200486 | - | 297 | NP_216467.1 | hypothetical protein | - |
| Rv1952 | 2200726..2200941 | + | 216 | NP_216468.1 | antitoxin VapB14 | - |
| Rv1953 | 2200938..2201249 | + | 312 | NP_216469.1 | ribonuclease VapC14 | - |
| Rv1954c | 2201223..2201744 | - | 522 | NP_216470.1 | hypothetical protein | - |
| Rv1955 | 2201719..2202096 | + | 378 | NP_216471.2 | toxin HigB | Toxin |
| Rv1956 | 2202138..2202587 | + | 450 | NP_216472.1 | antitoxin HigA | Antitoxin |
| Rv1957 | 2202584..2203129 | + | 546 | NP_216473.1 | SecB-like chaperone | - |
| Rv1958c | 2203018..2203632 | - | 615 | NP_216474.1 | hypothetical protein | - |
| Rv1959c | 2203681..2203977 | - | 297 | NP_216475.1 | toxin ParE1 | - |
| Rv1960c | 2203974..2204225 | - | 252 | NP_216476.1 | antitoxin ParD1 | - |
| Rv1961 | 2204212..2204706 | + | 495 | NP_216477.1 | hypothetical protein | - |
| Rv1962c | 2204866..2205273 | - | 408 | NP_216478.1 | ribonuclease VapC35 | - |
| Rv1962A | 2205277..2205549 | - | 273 | YP_007410641.1 | antitoxin VapB35 | - |
| Rv1963c | 2205582..2206802 | - | 1221 | NP_216479.1 | transcriptional repressor Mce3R | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 14397.77 Da Isoelectric Point: 10.8862
>T103 NP_216471.2 NC_000962:2201719-2202096 [Mycobacterium tuberculosis H37Rv]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI
Download Length: 378 bp
>T103 NC_000962:2201719-2202096 [Mycobacterium tuberculosis H37Rv]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA
Antitoxin
Download Length: 150 a.a. Molecular weight: 16760.16 Da Isoelectric Point: 8.0771
>AT103 NP_216472.1 NC_000962:2202138-2202587 [Mycobacterium tuberculosis H37Rv]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA
Download Length: 450 bp
>AT103 NC_000962:2202138-2202587 [Mycobacterium tuberculosis H37Rv]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Arun Sharma et al. (2021) HigB1 Toxin in Mycobacterium tuberculosis Is Upregulated During Stress and Required to Establish Infection in Guinea Pigs. Frontiers in Microbiology 12:748890. [PubMed:34917044]
(2) Dorothée L Schuessler et al. (2013) Induced ectopic expression of HigB toxin in Mycobacterium tuberculosis results in growth inhibition, reduced abundance of a subset of mRNAs and cleavage of tmRNA. Molecular Microbiology 90(1):195-207. [PubMed:23927792]
(3) Amanda S Fivian-Hughes et al. (2010) Analyzing the regulatory role of the HigA antitoxin within Mycobacterium tuberculosis. Journal of Bacteriology 192(17):4348-56. [PubMed:20585061]
(4) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113]
(5) Amita Gupta (2009) Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiology Letters 290(1):45-53. [PubMed:19016878]
(6) Pauline Texier et al. (2021) ClpXP-mediated Degradation of the TAC Antitoxin is Neutralized by the SecB-like Chaperone in Mycobacterium tuberculosis. Journal of Molecular Biology 433(5):166815. [PubMed:33450247]