Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1142690..1143384 Replicon chromosome
Accession NZ_CP028703
Organism Escherichia coli strain ME8067

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag DBB47_RS05810 Protein ID WP_001263489.1
Coordinates 1142986..1143384 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag DBB47_RS05805 Protein ID WP_000554757.1
Coordinates 1142690..1142983 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBB47_RS05785 1138322..1138819 + 498 WP_108118599.1 REP-associated tyrosine transposase RayT -
DBB47_RS05790 1139043..1140755 - 1713 Protein_1061 flagellar biosynthesis protein FlhA -
DBB47_RS05795 1140727..1141512 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
DBB47_RS05800 1141583..1142638 + 1056 WP_001226164.1 DNA polymerase IV -
DBB47_RS05805 1142690..1142983 + 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
DBB47_RS05810 1142986..1143384 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
DBB47_RS05815 1143394..1143846 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
DBB47_RS05820 1144164..1144370 + 207 Protein_1067 RtcB family protein -
DBB47_RS05825 1144366..1144887 + 522 Protein_1068 peptide chain release factor H -
DBB47_RS05830 1144944..1146401 - 1458 WP_108118600.1 cytosol nonspecific dipeptidase -
DBB47_RS05835 1146662..1147120 + 459 WP_108118601.1 xanthine phosphoribosyltransferase -
- 1147716..1147796 + 81 NuclAT_12 - -
- 1147716..1147796 + 81 NuclAT_12 - -
- 1147716..1147796 + 81 NuclAT_12 - -
- 1147716..1147796 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T102988 WP_001263489.1 NZ_CP028703:1142986-1143384 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T102988 NZ_CP028703:1142986-1143384 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT102988 WP_000554757.1 NZ_CP028703:1142690-1142983 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT102988 NZ_CP028703:1142690-1142983 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References