Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1136646..1137187 Replicon chromosome
Accession NZ_CP028703
Organism Escherichia coli strain ME8067

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag DBB47_RS05770 Protein ID WP_000615983.1
Coordinates 1136646..1136924 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag DBB47_RS05775 Protein ID WP_000729703.1
Coordinates 1136927..1137187 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBB47_RS05755 1134228..1134806 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
DBB47_RS05760 1135012..1135779 + 768 WP_000333380.1 class II glutamine amidotransferase -
DBB47_RS05765 1135750..1136490 - 741 WP_001225679.1 murein L,D-transpeptidase -
DBB47_RS05770 1136646..1136924 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
DBB47_RS05775 1136927..1137187 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
DBB47_RS05780 1137397..1138146 + 750 WP_000056849.1 C40 family peptidase -
DBB47_RS05785 1138322..1138819 + 498 WP_108118599.1 REP-associated tyrosine transposase RayT -
DBB47_RS05790 1139043..1140755 - 1713 Protein_1061 flagellar biosynthesis protein FlhA -
DBB47_RS05795 1140727..1141512 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T102987 WP_000615983.1 NZ_CP028703:c1136924-1136646 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T102987 NZ_CP028703:c1136924-1136646 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT102987 WP_000729703.1 NZ_CP028703:c1137187-1136927 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT102987 NZ_CP028703:c1137187-1136927 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References