102968

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2521763..2522265 Replicon chromosome
Accession NZ_CP028702
Organism Escherichia coli strain J53

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DBB46_RS13370 Protein ID WP_000767829.1
Coordinates 2521763..2522017 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DBB46_RS13375 Protein ID WP_001259255.1
Coordinates 2522014..2522265 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBB46_RS13340 2516778..2517005 - 228 WP_000234896.1 sulfurtransferase TusA -
DBB46_RS13345 2517019..2518077 - 1059 WP_000492339.1 transport protein YeeE -
DBB46_RS13350 2518256..2519614 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DBB46_RS13355 2519604..2519666 - 63 WP_010723108.1 membrane protein YoeI -
DBB46_RS13360 2519881..2520810 - 930 WP_000803351.1 LysR family transcriptional regulator -
DBB46_RS13365 2520856..2521680 - 825 WP_000754737.1 SDR family oxidoreductase -
DBB46_RS13370 2521763..2522017 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DBB46_RS13375 2522014..2522265 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DBB46_RS13380 2522548..2522598 + 51 WP_001364200.1 his operon leader peptide -
DBB46_RS13390 2522744..2523643 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DBB46_RS13395 2523649..2524953 + 1305 WP_000009594.1 histidinol dehydrogenase -
DBB46_RS13400 2524950..2526020 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
DBB46_RS13405 2526020..2527087 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T102968 WP_000767829.1 NZ_CP028702:c2522017-2521763 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T102968 NZ_CP028702:c2522017-2521763 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT102968 WP_001259255.1 NZ_CP028702:c2522265-2522014 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT102968 NZ_CP028702:c2522265-2522014 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References