Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2521763..2522265 Replicon chromosome
Accession NZ_CP028702
Organism Escherichia coli strain J53

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DBB46_RS13370 Protein ID WP_000767829.1
Coordinates 2521763..2522017 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DBB46_RS13375 Protein ID WP_001259255.1
Coordinates 2522014..2522265 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBB46_RS13340 2516778..2517005 - 228 WP_000234896.1 sulfurtransferase TusA -
DBB46_RS13345 2517019..2518077 - 1059 WP_000492339.1 transport protein YeeE -
DBB46_RS13350 2518256..2519614 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DBB46_RS13355 2519604..2519666 - 63 WP_010723108.1 membrane protein YoeI -
DBB46_RS13360 2519881..2520810 - 930 WP_000803351.1 LysR family transcriptional regulator -
DBB46_RS13365 2520856..2521680 - 825 WP_000754737.1 SDR family oxidoreductase -
DBB46_RS13370 2521763..2522017 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DBB46_RS13375 2522014..2522265 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DBB46_RS13380 2522548..2522598 + 51 WP_001364200.1 his operon leader peptide -
DBB46_RS13390 2522744..2523643 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
DBB46_RS13395 2523649..2524953 + 1305 WP_000009594.1 histidinol dehydrogenase -
DBB46_RS13400 2524950..2526020 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
DBB46_RS13405 2526020..2527087 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T102968 WP_000767829.1 NZ_CP028702:c2522017-2521763 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T102968 NZ_CP028702:c2522017-2521763 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT102968 WP_001259255.1 NZ_CP028702:c2522265-2522014 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT102968 NZ_CP028702:c2522265-2522014 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References