Detailed information of TA system TA102729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4477438..4478132
• Replicon: chromosome
• Accession: NZ_CP028685
• Organism: Escherichia coli strain 111

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: C9E40_RS23405
• Protein ID: WP_001263495.1
• Coordinates: 4477438..4477836 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: C9E40_RS23410
• Protein ID: WP_000554757.1
• Coordinates: 4477839..4478132 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4473025..4473105	-	81	NuclAT_12	-	-
-	4473025..4473105	-	81	NuclAT_12	-	-
-	4473025..4473105	-	81	NuclAT_12	-	-
-	4473025..4473105	-	81	NuclAT_12	-	-
C9E40_RS23380	4473701..4474159	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
C9E40_RS23385	4474420..4475877	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
C9E40_RS23390	4475934..4476455	-	522	Protein_4446	peptide chain release factor H	-
C9E40_RS23395	4476454..4476657	-	204	Protein_4447	RNA ligase RtcB family protein	-
C9E40_RS23400	4476976..4477428	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
C9E40_RS23405	4477438..4477836	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C9E40_RS23410	4477839..4478132	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C9E40_RS23415	4478184..4479239	-	1056	WP_001226188.1	DNA polymerase IV	-
C9E40_RS23420	4479310..4480095	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
C9E40_RS23425	4480067..4481779	+	1713	Protein_4453	flagellar biosynthesis protein FlhA	-
C9E40_RS23430	4481924..4482421	-	498	Protein_4454	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T102729 WP_001263495.1 NZ_CP028685:c4477836-4477438 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T102729 NZ_CP028685:c4477836-4477438 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT102729 WP_000554757.1 NZ_CP028685:c4478132-4477839 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT102729 NZ_CP028685:c4478132-4477839 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6