Detailed information of TA system TA102595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4318276..4318970
• Replicon: chromosome
• Accession: NZ_CP028674
• Organism: Escherichia coli strain 115

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: C9E44_RS22170
• Protein ID: WP_001263495.1
• Coordinates: 4318276..4318674 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: C9E44_RS22175
• Protein ID: WP_000554757.1
• Coordinates: 4318677..4318970 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4313863..4313943	-	81	NuclAT_11	-	-
-	4313863..4313943	-	81	NuclAT_11	-	-
-	4313863..4313943	-	81	NuclAT_11	-	-
-	4313863..4313943	-	81	NuclAT_11	-	-
C9E44_RS22145	4314539..4314997	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
C9E44_RS22150	4315258..4316715	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
C9E44_RS22155	4316772..4317293	-	522	Protein_4218	peptide chain release factor H	-
C9E44_RS22160	4317292..4317495	-	204	Protein_4219	RNA ligase RtcB family protein	-
C9E44_RS22165	4317814..4318266	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
C9E44_RS22170	4318276..4318674	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C9E44_RS22175	4318677..4318970	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C9E44_RS22180	4319022..4320077	-	1056	WP_001226186.1	DNA polymerase IV	-
C9E44_RS22185	4320148..4320933	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
C9E44_RS22190	4320905..4322617	+	1713	Protein_4225	flagellar biosynthesis protein FlhA	-
C9E44_RS22195	4322762..4323259	-	498	Protein_4226	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4252484..4324208	71724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T102595 WP_001263495.1 NZ_CP028674:c4318674-4318276 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T102595 NZ_CP028674:c4318674-4318276 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT102595 WP_000554757.1 NZ_CP028674:c4318970-4318677 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT102595 NZ_CP028674:c4318970-4318677 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6