Detailed information of TA system TA102514

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2586739..2586964
• Replicon: chromosome
• Accession: NZ_CP028668
• Organism: Escherichia coli strain 117

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: C9E46_RS13385
• Protein ID: WP_000813258.1
• Coordinates: 2586809..2586964 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2586739..2586797 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E46_RS13335	2581804..2582046	+	243	WP_000747948.1	hypothetical protein	-
C9E46_RS13340	2582030..2582455	+	426	WP_000693878.1	Rha family transcriptional regulator	-
C9E46_RS13345	2582524..2583567	+	1044	WP_001262402.1	hypothetical protein	-
C9E46_RS13350	2583560..2584021	+	462	WP_000139447.1	replication protein	-
C9E46_RS13355	2584055..2584771	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
C9E46_RS13360	2584804..2585085	+	282	WP_000603384.1	DNA-binding protein	-
C9E46_RS13365	2585082..2585309	+	228	WP_000699809.1	hypothetical protein	-
C9E46_RS13370	2585302..2585613	+	312	WP_001289673.1	hypothetical protein	-
C9E46_RS13375	2585741..2585959	+	219	WP_000683609.1	DUF4014 family protein	-
C9E46_RS13380	2585961..2586518	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2586739..2586797	-	59	-	-	Antitoxin
C9E46_RS13385	2586809..2586964	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
C9E46_RS13390	2587084..2587428	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
C9E46_RS13395	2587550..2587822	+	273	WP_000191872.1	hypothetical protein	-
C9E46_RS13400	2587824..2588873	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
C9E46_RS13405	2588886..2589191	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
C9E46_RS13410	2589254..2589808	+	555	WP_000640035.1	DUF1133 family protein	-
C9E46_RS13415	2590033..2590230	+	198	WP_000917763.1	hypothetical protein	-
C9E46_RS13420	2590366..2591079	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
C9E46_RS13435	2591530..2591961	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1	2507154..2624078	116924

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T102514 WP_000813258.1 NZ_CP028668:2586809-2586964 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T102514 NZ_CP028668:2586809-2586964 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT102514 NZ_CP028668:c2586797-2586739 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin