Detailed information of TA system TA102452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 4381717..4382256
• Replicon: chromosome
• Accession: NZ_CP028662
• Organism: Escherichia coli strain 119

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: C9E48_RS22665
• Protein ID: WP_001303998.1
• Coordinates: 4381996..4382256 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: C9E48_RS22660
• Protein ID: WP_000729705.1
• Coordinates: 4381717..4381977 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E48_RS22640	4377471..4378256	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
C9E48_RS22645	4378228..4379940	+	1713	Protein_4306	flagellar biosynthesis protein FlhA	-
C9E48_RS22650	4380085..4380582	-	498	Protein_4307	REP-associated tyrosine transposase RayT	-
C9E48_RS22655	4380758..4381531	-	774	WP_000543891.1	C40 family peptidase	-
C9E48_RS22660	4381717..4381977	+	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
C9E48_RS22665	4381996..4382256	+	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
C9E48_RS22670	4382412..4383152	+	741	WP_001225679.1	murein L,D-transpeptidase	-
C9E48_RS22675	4383123..4383890	-	768	WP_001301698.1	class II glutamine amidotransferase	-
C9E48_RS22680	4383995..4384573	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T102452 WP_001303998.1 NZ_CP028662:4381996-4382256 [Escherichia coli]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T102452 NZ_CP028662:4381996-4382256 [Escherichia coli]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT102452 WP_000729705.1 NZ_CP028662:4381717-4381977 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT102452 NZ_CP028662:4381717-4381977 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA