Detailed information of TA system TA102343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4424668..4425362
• Replicon: chromosome
• Accession: NZ_CP028654
• Organism: Escherichia coli strain 122

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: C9E51_RS22995
• Protein ID: WP_001263495.1
• Coordinates: 4424668..4425066 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: C9E51_RS23000
• Protein ID: WP_000554757.1
• Coordinates: 4425069..4425362 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4420255..4420335	-	81	NuclAT_12	-	-
-	4420255..4420335	-	81	NuclAT_12	-	-
-	4420255..4420335	-	81	NuclAT_12	-	-
-	4420255..4420335	-	81	NuclAT_12	-	-
C9E51_RS22970	4420931..4421389	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
C9E51_RS22975	4421650..4423107	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
C9E51_RS22980	4423164..4423685	-	522	Protein_4372	peptide chain release factor H	-
C9E51_RS22985	4423684..4423887	-	204	Protein_4373	RNA ligase RtcB family protein	-
C9E51_RS22990	4424206..4424658	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
C9E51_RS22995	4424668..4425066	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C9E51_RS23000	4425069..4425362	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C9E51_RS23005	4425414..4426469	-	1056	WP_001226188.1	DNA polymerase IV	-
C9E51_RS23010	4426540..4427325	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
C9E51_RS23015	4427297..4429009	+	1713	Protein_4379	flagellar biosynthesis protein FlhA	-
C9E51_RS23020	4429154..4429651	-	498	Protein_4380	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T102343 WP_001263495.1 NZ_CP028654:c4425066-4424668 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T102343 NZ_CP028654:c4425066-4424668 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT102343 WP_000554757.1 NZ_CP028654:c4425362-4425069 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT102343 NZ_CP028654:c4425362-4425069 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6