Detailed information of TA system TA102102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4375679..4376373
• Replicon: chromosome
• Accession: NZ_CP028635
• Organism: Escherichia coli strain 134

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: C9E58_RS22625
• Protein ID: WP_001263495.1
• Coordinates: 4375679..4376077 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: C9E58_RS22630
• Protein ID: WP_000554757.1
• Coordinates: 4376080..4376373 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4371266..4371346	-	81	NuclAT_11	-	-
-	4371266..4371346	-	81	NuclAT_11	-	-
-	4371266..4371346	-	81	NuclAT_11	-	-
-	4371266..4371346	-	81	NuclAT_11	-	-
C9E58_RS22600	4371942..4372400	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
C9E58_RS22605	4372661..4374118	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
C9E58_RS22610	4374175..4374696	-	522	Protein_4300	peptide chain release factor H	-
C9E58_RS22615	4374695..4374898	-	204	Protein_4301	RNA ligase RtcB family protein	-
C9E58_RS22620	4375217..4375669	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
C9E58_RS22625	4375679..4376077	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C9E58_RS22630	4376080..4376373	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C9E58_RS22635	4376425..4377480	-	1056	WP_001226186.1	DNA polymerase IV	-
C9E58_RS22640	4377551..4378336	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
C9E58_RS22645	4378308..4380020	+	1713	Protein_4307	flagellar biosynthesis protein FlhA	-
C9E58_RS22650	4380165..4380662	-	498	Protein_4308	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T102102 WP_001263495.1 NZ_CP028635:c4376077-4375679 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T102102 NZ_CP028635:c4376077-4375679 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT102102 WP_000554757.1 NZ_CP028635:c4376373-4376080 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT102102 NZ_CP028635:c4376373-4376080 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6