Detailed information of TA system TA101986

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2586736..2586961
• Replicon: chromosome
• Accession: NZ_CP028626
• Organism: Escherichia coli strain 137

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: C9E61_RS13385
• Protein ID: WP_000813258.1
• Coordinates: 2586806..2586961 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2586736..2586794 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E61_RS13335	2581801..2582043	+	243	WP_000747948.1	hypothetical protein	-
C9E61_RS13340	2582027..2582452	+	426	WP_000693878.1	Rha family transcriptional regulator	-
C9E61_RS13345	2582521..2583564	+	1044	WP_001262402.1	hypothetical protein	-
C9E61_RS13350	2583557..2584018	+	462	WP_000139447.1	replication protein	-
C9E61_RS13355	2584052..2584768	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
C9E61_RS13360	2584801..2585082	+	282	WP_000603384.1	DNA-binding protein	-
C9E61_RS13365	2585079..2585306	+	228	WP_000699809.1	hypothetical protein	-
C9E61_RS13370	2585299..2585610	+	312	WP_001289673.1	hypothetical protein	-
C9E61_RS13375	2585738..2585956	+	219	WP_000683609.1	DUF4014 family protein	-
C9E61_RS13380	2585958..2586515	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2586736..2586794	-	59	-	-	Antitoxin
C9E61_RS13385	2586806..2586961	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
C9E61_RS13390	2587081..2587425	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
C9E61_RS13395	2587547..2587819	+	273	WP_000191872.1	hypothetical protein	-
C9E61_RS13400	2587821..2588870	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
C9E61_RS13405	2588883..2589188	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
C9E61_RS13410	2589251..2589805	+	555	WP_000640035.1	DUF1133 family protein	-
C9E61_RS13415	2590030..2590227	+	198	WP_000917763.1	hypothetical protein	-
C9E61_RS13420	2590363..2591076	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
C9E61_RS13435	2591527..2591958	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1	2502025..2624075	122050

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T101986 WP_000813258.1 NZ_CP028626:2586806-2586961 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T101986 NZ_CP028626:2586806-2586961 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT101986 NZ_CP028626:c2586794-2586736 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin