101887

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4377141..4377835 Replicon chromosome
Accession NZ_CP028617
Organism Escherichia coli strain 140

Toxin (Protein)


Gene name yafO Uniprot ID C3TNX7
Locus tag C9E64_RS22640 Protein ID WP_001263495.1
Coordinates 4377141..4377539 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag C9E64_RS22645 Protein ID WP_000554757.1
Coordinates 4377542..4377835 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 4372728..4372808 - 81 NuclAT_11 - -
- 4372728..4372808 - 81 NuclAT_11 - -
- 4372728..4372808 - 81 NuclAT_11 - -
- 4372728..4372808 - 81 NuclAT_11 - -
C9E64_RS22615 4373404..4373862 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
C9E64_RS22620 4374123..4375580 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
C9E64_RS22625 4375637..4376158 - 522 Protein_4302 peptide chain release factor H -
C9E64_RS22630 4376157..4376360 - 204 Protein_4303 RNA ligase RtcB family protein -
C9E64_RS22635 4376679..4377131 - 453 WP_001059871.1 GNAT family N-acetyltransferase -
C9E64_RS22640 4377141..4377539 - 399 WP_001263495.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
C9E64_RS22645 4377542..4377835 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
C9E64_RS22650 4377887..4378942 - 1056 WP_001226186.1 DNA polymerase IV -
C9E64_RS22655 4379013..4379798 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
C9E64_RS22660 4379770..4381482 + 1713 Protein_4309 flagellar biosynthesis protein FlhA -
C9E64_RS22665 4381627..4382124 - 498 Protein_4310 REP-associated tyrosine transposase RayT -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15473.86 Da        Isoelectric Point: 8.0949

>T101887 WP_001263495.1 NZ_CP028617:c4377539-4377141 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T101887 NZ_CP028617:c4377539-4377141 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT101887 WP_000554757.1 NZ_CP028617:c4377835-4377542 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT101887 NZ_CP028617:c4377835-4377542 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References