Detailed information of TA system TA010186

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/-
• Location: 50278..51889
• Replicon: plasmid pVbh_BscR1
• Accession: NZ_CP019790
• Organism: Bartonella schoenbuchensis R1

Toxin (Protein)

• Gene name: VbhT
• Uniprot ID: -
• Locus tag: BscR1v2_RS07925
• Protein ID: WP_153302026.1
• Coordinates: 50278..51714 (-)
• Length: 479 a.a.

Antitoxin (Protein)

• Gene name: VbhA
• Uniprot ID: -
• Locus tag: BscR1v2_RS07930
• Protein ID: WP_078690388.1
• Coordinates: 51701..51889 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BscR1v2_RS07890 (BscR1v2_016850)	45504..45791	+	288	WP_078690383.1	type IV secretion protein VblB7	-
BscR1v2_RS07895 (BscR1v2_016860)	45797..46468	+	672	WP_078690384.1	virB8 family protein	-
BscR1v2_RS07900 (BscR1v2_016870)	46471..47325	+	855	WP_078690385.1	P-type conjugative transfer protein VirB9	-
BscR1v2_RS07905 (BscR1v2_016880)	47240..48481	+	1242	WP_078690386.1	type IV secretion system protein VirB10	-
BscR1v2_RS07910 (BscR1v2_016890)	48494..49546	+	1053	WP_010703103.1	P-type DNA transfer ATPase VirB11	-
BscR1v2_RS07915 (BscR1v2_016900)	49569..49883	-	315	WP_010703104.1	helix-turn-helix domain-containing protein	-
BscR1v2_RS07920 (BscR1v2_016910)	49883..50224	-	342	WP_078690387.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BscR1v2_RS07925 (BscR1v2_016920)	50278..51714	-	1437	WP_153302026.1	Fic family protein	Toxin
BscR1v2_RS07930 (BscR1v2_016930)	51701..51889	-	189	WP_078690388.1	antitoxin VbhA family protein	Antitoxin
BscR1v2_RS07935 (BscR1v2_016940)	51972..55691	-	3720	WP_078690389.1	Ti-type conjugative transfer relaxase TraA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	virB4 / virB11	1..55761	55761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bartonella effector protein, BID domain (259-349)

Antitoxin

• Antitoxin VbhA (10-53)

Sequences

Toxin

Protein

• Length: 479 a.a.
• Molecular weight: 55346.66 Da
• Isoelectric Point: 9.8741

>T10186 WP_153302026.1 NZ_CP019790:c51714-50278 [Bartonella schoenbuchensis R1]
VRKYEGSNDPYTDPETGVMYNLLGIKDQARLERVESAFAYIRSFELGRTSISGKFDLDHMKKIHKKLFGDVYEWAGKTRL
VDIVKDNSKFAHYTQIESYAPQITQQLAREQHLRGLDANEFSQRAGYYMGELNALHPFREGNGRTLREFIWQLAREAGYH
IDWDRVERQEMTRASIESYYGNSDLMSALIRRNLTEFTVNRRVDVSQGINERVLSHIDIDKEWPQKGFNIAIQTTQQAPY
LSSYTDTSNLEEKAQNALRNEQSYVDTFKELNDHLKTIYKDPQAAALKIEQTILAGKGDKLPDILAKAPNKVGELRGSDR
LIDKLKSAGKERKAALYNVPLAISTIRRLQSFYKNSYEKHMDKLTREREQLKVEVPSLSQEAVAYMKNVEVGRNNYSKIP
ENINKEFVQLESALNRRFGKDVIYKRNFNLSKEIASKQTYDKKLVNELQTAIKFLQQRHIQKQNNLAITRTPSKGITR

Nucleotide

• Length: 1437 bp

>T10186 NZ_CP019790:c51714-50278 [Bartonella schoenbuchensis R1]
GTGAGGAAATATGAGGGTAGCAACGATCCTTATACTGATCCAGAAACTGGTGTTATGTATAATCTGCTTGGAATTAAAGA
CCAAGCCAGATTGGAGCGTGTAGAATCTGCTTTCGCTTATATAAGGTCTTTTGAGCTTGGGCGTACTTCTATTAGCGGTA
AGTTCGATCTAGATCACATGAAAAAAATCCACAAAAAGCTGTTTGGTGACGTCTATGAATGGGCAGGAAAGACTCGTTTA
GTGGATATTGTCAAAGATAATAGTAAATTTGCTCATTATACTCAAATTGAAAGTTACGCACCGCAGATTACACAACAGCT
TGCAAGAGAACAACATTTACGTGGTCTTGACGCTAATGAATTTAGCCAGAGGGCTGGATATTACATGGGCGAATTGAATG
CGTTGCACCCGTTTCGAGAAGGTAATGGACGTACTTTACGTGAATTTATCTGGCAACTTGCTCGTGAAGCTGGTTATCAT
ATCGACTGGGATCGTGTAGAGCGACAAGAAATGACGCGTGCGTCAATTGAATCATATTATGGCAACTCTGATTTAATGTC
TGCGCTAATCCGTAGAAACCTCACTGAATTTACAGTAAATAGAAGAGTTGATGTTTCTCAAGGTATTAATGAACGAGTTC
TTTCTCACATTGATATAGATAAAGAATGGCCACAAAAAGGTTTTAATATAGCAATACAAACAACACAACAGGCACCATAT
CTTTCTTCTTATACAGACACTTCTAATCTAGAAGAGAAAGCGCAAAACGCTCTTAGAAATGAACAATCTTATGTTGATAC
TTTTAAAGAATTAAACGATCATTTGAAGACTATCTATAAGGATCCCCAAGCAGCTGCACTTAAGATTGAACAAACAATTT
TAGCAGGCAAGGGGGATAAATTACCTGATATTTTAGCTAAAGCACCTAATAAAGTTGGTGAACTGCGCGGATCAGACCGT
TTAATAGACAAACTAAAATCAGCAGGAAAAGAACGAAAAGCTGCACTTTATAATGTTCCTCTTGCAATTTCTACGATTAG
AAGACTTCAATCATTTTATAAAAATTCCTATGAGAAGCATATGGACAAACTTACACGTGAACGTGAACAATTAAAGGTTG
AAGTGCCTTCTCTCTCTCAAGAAGCTGTCGCTTATATGAAAAATGTTGAAGTTGGCCGTAATAATTATTCCAAGATACCA
GAGAACATTAATAAAGAATTTGTGCAGCTTGAAAGTGCTTTAAATAGGCGTTTTGGAAAGGATGTAATCTATAAGCGAAA
TTTTAACTTATCAAAAGAAATAGCCTCAAAACAGACTTATGATAAAAAGCTTGTCAATGAACTTCAAACGGCAATAAAAT
TTCTACAACAAAGACATATACAAAAACAAAACAATCTTGCGATAACACGCACGCCTTCAAAGGGAATTACAAGGTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 7270.24 Da
• Isoelectric Point: 4.2939

>AT10186 WP_078690388.1 NZ_CP019790:c51889-51701 [Bartonella schoenbuchensis R1]
MLSEEEIEYRRRDARNALASQRLEGLEPDPQVVAQMERVVVGELETSDVIKDLMERIKREEI

Nucleotide

• Length: 189 bp

>AT10186 NZ_CP019790:c51889-51701 [Bartonella schoenbuchensis R1]
ATGTTGAGCGAGGAAGAAATCGAATACCGTCGCCGTGATGCTAGAAATGCGTTGGCTAGTCAACGTTTAGAGGGTTTGGA
ACCAGATCCACAAGTCGTTGCACAAATGGAGCGTGTTGTTGTTGGAGAGCTTGAAACAAGTGATGTAATCAAGGATTTGA
TGGAACGGATAAAACGTGAGGAAATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10184	Campylobacter fetus subsp. venerealis str. 84-112	35.185	96.861	0.341

Multiple sequence alignment

References

(1) Alexander Harms et al. (2017) A bacterial toxin-antitoxin module is the origin of inter-bacterial and inter-kingdom effectors of Bartonella. PLoS Genetics 13(10):e1007077. [PubMed:29073136]


 experimental literature