Detailed information of TA system TA101842

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2586374..2586599
• Replicon: chromosome
• Accession: NZ_CP028614
• Organism: Escherichia coli strain 141

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: C9E65_RS13380
• Protein ID: WP_000813258.1
• Coordinates: 2586444..2586599 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2586374..2586432 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E65_RS13330	2581439..2581681	+	243	WP_000747948.1	hypothetical protein	-
C9E65_RS13335	2581665..2582090	+	426	WP_000693878.1	Rha family transcriptional regulator	-
C9E65_RS13340	2582159..2583202	+	1044	WP_001262402.1	hypothetical protein	-
C9E65_RS13345	2583195..2583656	+	462	WP_000139447.1	replication protein	-
C9E65_RS13350	2583690..2584406	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
C9E65_RS13355	2584439..2584720	+	282	WP_000603384.1	DNA-binding protein	-
C9E65_RS13360	2584717..2584944	+	228	WP_000699809.1	hypothetical protein	-
C9E65_RS13365	2584937..2585248	+	312	WP_001289673.1	hypothetical protein	-
C9E65_RS13370	2585376..2585594	+	219	WP_000683609.1	DUF4014 family protein	-
C9E65_RS13375	2585596..2586153	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2586374..2586432	-	59	-	-	Antitoxin
C9E65_RS13380	2586444..2586599	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
C9E65_RS13385	2586719..2587063	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
C9E65_RS13390	2587185..2587457	+	273	WP_000191872.1	hypothetical protein	-
C9E65_RS13395	2587459..2588508	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
C9E65_RS13400	2588521..2588826	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
C9E65_RS13405	2588889..2589443	+	555	WP_000640035.1	DUF1133 family protein	-
C9E65_RS13410	2589668..2589865	+	198	WP_000917763.1	hypothetical protein	-
C9E65_RS13415	2590001..2590714	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
C9E65_RS13430	2591165..2591596	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1	2513381..2623713	110332

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T101842 WP_000813258.1 NZ_CP028614:2586444-2586599 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T101842 NZ_CP028614:2586444-2586599 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT101842 NZ_CP028614:c2586432-2586374 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin