Detailed information of TA system TA101817

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 23981..24401
• Replicon: plasmid pTA142-3
• Accession: NZ_CP028610
• Organism: Escherichia coli strain 142

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: C9E66_RS27800
• Protein ID: WP_000775238.1
• Coordinates: 24240..24401 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	23981..24175 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E66_RS27765	19665..20186	+	522	WP_000290783.1	single-stranded DNA-binding protein	-
C9E66_RS27775	20244..20477	+	234	WP_069192978.1	DUF905 domain-containing protein	-
C9E66_RS27780	20538..22502	+	1965	WP_137052466.1	ParB/RepB/Spo0J family partition protein	-
C9E66_RS27785	22571..23005	+	435	WP_000845923.1	conjugation system SOS inhibitor PsiB	-
C9E66_RS27790	23002..23721	+	720	WP_001276237.1	plasmid SOS inhibition protein A	-
C9E66_RS27795	23718..24032	+	315	WP_000872084.1	hypothetical protein	-
-	23981..24175	+	195	NuclAT_0	-	Antitoxin
-	23981..24175	+	195	NuclAT_0	-	Antitoxin
-	23981..24175	+	195	NuclAT_0	-	Antitoxin
-	23981..24175	+	195	NuclAT_0	-	Antitoxin
C9E66_RS27800	24240..24401	+	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
C9E66_RS27805	24702..24999	-	298	Protein_34	hypothetical protein	-
C9E66_RS27820	25296..25598	+	303	WP_001272235.1	hypothetical protein	-
C9E66_RS27830	25754..26041	+	288	WP_000107543.1	hypothetical protein	-
C9E66_RS27835	26160..26981	+	822	WP_032107531.1	DUF945 domain-containing protein	-
C9E66_RS27840	27278..27880	-	603	WP_077785958.1	transglycosylase SLT domain-containing protein	-
C9E66_RS27845	28220..28603	+	384	WP_032210979.1	relaxosome protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..67786	67786

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T101817 WP_000775238.1 NZ_CP028610:24240-24401 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T101817 NZ_CP028610:24240-24401 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 195 bp

>AT101817 NZ_CP028610:23981-24175 [Escherichia coli]
ACTGATTGCCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGTGCGCAGGTATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin