Detailed information of TA system TA101812

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4748702..4749114
• Replicon: chromosome
• Accession: NZ_CP028609
• Organism: Escherichia coli strain 142

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: C9E66_RS24490
• Protein ID: WP_000132630.1
• Coordinates: 4748773..4749114 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4748702..4748778 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E66_RS24480	4745329..4746798	+	1470	WP_001302468.1	type I restriction-modification system subunit M	-
C9E66_RS24485	4746798..4748552	+	1755	WP_000110082.1	restriction endonuclease subunit S	-
-	4748702..4748778	-	77	NuclAT_15	-	Antitoxin
-	4748702..4748778	-	77	NuclAT_15	-	Antitoxin
-	4748702..4748778	-	77	NuclAT_15	-	Antitoxin
-	4748702..4748778	-	77	NuclAT_15	-	Antitoxin
-	4748702..4748778	-	77	NuclAT_16	-	Antitoxin
-	4748702..4748778	-	77	NuclAT_16	-	Antitoxin
-	4748702..4748778	-	77	NuclAT_16	-	Antitoxin
-	4748702..4748778	-	77	NuclAT_16	-	Antitoxin
C9E66_RS24490	4748773..4749114	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
C9E66_RS24495	4749161..4750324	-	1164	WP_001304006.1	DUF1524 domain-containing protein	-
C9E66_RS24500	4750372..4751253	-	882	WP_001304007.1	DUF262 domain-containing protein	-
C9E66_RS24505	4751350..4751514	-	165	WP_000394274.1	DUF1127 domain-containing protein	-
C9E66_RS24510	4751691..4753103	+	1413	WP_000199295.1	PLP-dependent aminotransferase family protein	-
C9E66_RS29445	4753346..4753546	-	201	WP_001310455.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002655 Escherichia coli O157:H7 str. EDL933 5456776..5475497 0.003 View NC_002695 Escherichia coli O157:H7 str. Sakai 5426911..5445631 0.003	inside	Genomic island	-	espX6	4740901..4762140	21239

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T101812 WP_000132630.1 NZ_CP028609:4748773-4749114 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T101812 NZ_CP028609:4748773-4749114 [Escherichia coli]
ATGACTGATACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGTTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTTGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT101812 NZ_CP028609:c4748778-4748702 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin