Detailed information of TA system TA101737

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2678380..2678605
• Replicon: chromosome
• Accession: NZ_CP028603
• Organism: Escherichia coli strain 144

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: C9E68_RS13950
• Protein ID: WP_000813258.1
• Coordinates: 2678450..2678605 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2678380..2678438 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E68_RS13900	2673445..2673687	+	243	WP_000747948.1	hypothetical protein	-
C9E68_RS13905	2673671..2674096	+	426	WP_000693878.1	Rha family transcriptional regulator	-
C9E68_RS13910	2674165..2675208	+	1044	WP_001262402.1	hypothetical protein	-
C9E68_RS13915	2675201..2675662	+	462	WP_000139447.1	replication protein	-
C9E68_RS13920	2675696..2676412	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
C9E68_RS13925	2676445..2676726	+	282	WP_000603384.1	DNA-binding protein	-
C9E68_RS13930	2676723..2676950	+	228	WP_000699809.1	hypothetical protein	-
C9E68_RS13935	2676943..2677254	+	312	WP_001289673.1	hypothetical protein	-
C9E68_RS13940	2677382..2677600	+	219	WP_137049466.1	DUF4014 family protein	-
C9E68_RS13945	2677602..2678159	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2678380..2678438	-	59	-	-	Antitoxin
C9E68_RS13950	2678450..2678605	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
C9E68_RS13955	2678725..2679069	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
C9E68_RS13960	2679191..2679463	+	273	WP_000191872.1	hypothetical protein	-
C9E68_RS13965	2679465..2680514	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
C9E68_RS13970	2680527..2680832	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
C9E68_RS13975	2680895..2681449	+	555	WP_000640035.1	DUF1133 family protein	-
C9E68_RS13980	2681674..2681871	+	198	WP_000917763.1	hypothetical protein	-
C9E68_RS13985	2682008..2682721	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
C9E68_RS14000	2683176..2683604	+	429	WP_001303509.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1 / nleG7'	2551493..2715721	164228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T101737 WP_000813258.1 NZ_CP028603:2678450-2678605 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T101737 NZ_CP028603:2678450-2678605 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT101737 NZ_CP028603:c2678438-2678380 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin