Detailed information of TA system TA101735

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2628988..2629213
• Replicon: chromosome
• Accession: NZ_CP028603
• Organism: Escherichia coli strain 144

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: C9E68_RS13615
• Protein ID: WP_000813254.1
• Coordinates: 2629058..2629213 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2628988..2629046 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E68_RS13570	2624241..2625194	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
C9E68_RS13575	2625201..2625941	+	741	WP_000790456.1	ATP-binding protein	-
C9E68_RS13580	2625971..2626741	+	771	WP_000450888.1	DUF1627 domain-containing protein	-
C9E68_RS13585	2626757..2627152	+	396	WP_001118161.1	DUF977 family protein	-
C9E68_RS13590	2627209..2627565	+	357	WP_001302146.1	hypothetical protein	-
C9E68_RS13595	2627614..2627826	+	213	WP_000063625.1	hypothetical protein	-
C9E68_RS13600	2627862..2628233	+	372	WP_000137941.1	hypothetical protein	-
C9E68_RS13605	2628230..2628592	+	363	WP_000610379.1	DUF551 domain-containing protein	-
C9E68_RS13610	2628708..2628812	+	105	WP_001278450.1	hypothetical protein	-
-	2628988..2629046	-	59	-	-	Antitoxin
C9E68_RS13615	2629058..2629213	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
C9E68_RS28975	2629510..2629677	+	168	WP_000998188.1	hypothetical protein	-
C9E68_RS13620	2629743..2630021	+	279	WP_001304183.1	hypothetical protein	-
C9E68_RS13625	2630023..2631072	+	1050	Protein_2604	DUF968 domain-containing protein	-
C9E68_RS13630	2631085..2631459	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
C9E68_RS13635	2631456..2632277	+	822	WP_000762904.1	antitermination protein	-
C9E68_RS13640	2632504..2632701	+	198	WP_000917735.1	hypothetical protein	-
C9E68_RS13645	2632852..2633910	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1 / nleG7'	2551493..2715721	164228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T101735 WP_000813254.1 NZ_CP028603:2629058-2629213 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T101735 NZ_CP028603:2629058-2629213 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT101735 NZ_CP028603:c2629046-2628988 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin