Detailed information of TA system TA101669

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3493258..3493483
• Replicon: chromosome
• Accession: NZ_CP028598
• Organism: Escherichia coli strain 148

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: C9E70_RS18430
• Protein ID: WP_000813263.1
• Coordinates: 3493258..3493413 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	3493425..3493483 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E70_RS18395	3488712..3489425	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
C9E70_RS18400	3489563..3489759	-	197	Protein_3507	TrmB family transcriptional regulator	-
C9E70_RS18405	3490046..3490864	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
C9E70_RS18410	3491016..3491387	-	372	WP_000090264.1	antitermination protein	-
C9E70_RS18415	3491377..3491748	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
C9E70_RS18420	3491761..3492810	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
C9E70_RS18425	3492812..3493090	-	279	WP_001341388.1	hypothetical protein	-
C9E70_RS18430	3493258..3493413	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	3493425..3493483	+	59	-	-	Antitoxin
C9E70_RS18450	3494018..3494791	+	774	WP_000160654.1	alpha/beta hydrolase	-
C9E70_RS18455	3495143..3495556	-	414	WP_001151233.1	DUF977 family protein	-
C9E70_RS18460	3495572..3496342	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
C9E70_RS18465	3496364..3497110	-	747	WP_000788745.1	ATP-binding protein	-
C9E70_RS18470	3497117..3498208	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T101669 WP_000813263.1 NZ_CP028598:c3493413-3493258 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T101669 NZ_CP028598:c3493413-3493258 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT101669 NZ_CP028598:3493425-3493483 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin