Detailed information of TA system TA101639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4379952..4380646
• Replicon: chromosome
• Accession: NZ_CP028596
• Organism: Escherichia coli strain 149

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: C9E71_RS22680
• Protein ID: WP_001263495.1
• Coordinates: 4379952..4380350 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: C9E71_RS22685
• Protein ID: WP_000554757.1
• Coordinates: 4380353..4380646 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4375539..4375619	-	81	NuclAT_11	-	-
-	4375539..4375619	-	81	NuclAT_11	-	-
-	4375539..4375619	-	81	NuclAT_11	-	-
-	4375539..4375619	-	81	NuclAT_11	-	-
C9E71_RS22655	4376215..4376673	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
C9E71_RS22660	4376934..4378391	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
C9E71_RS22665	4378448..4378969	-	522	Protein_4318	peptide chain release factor H	-
C9E71_RS22670	4378968..4379171	-	204	Protein_4319	RNA ligase RtcB family protein	-
C9E71_RS22675	4379490..4379942	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
C9E71_RS22680	4379952..4380350	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C9E71_RS22685	4380353..4380646	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C9E71_RS22690	4380698..4381753	-	1056	WP_001226187.1	DNA polymerase IV	-
C9E71_RS22695	4381824..4382609	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
C9E71_RS22700	4382581..4384293	+	1713	Protein_4325	flagellar biosynthesis protein FlhA	-
C9E71_RS22705	4384438..4384935	-	498	Protein_4326	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T101639 WP_001263495.1 NZ_CP028596:c4380350-4379952 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T101639 NZ_CP028596:c4380350-4379952 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT101639 WP_000554757.1 NZ_CP028596:c4380646-4380353 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT101639 NZ_CP028596:c4380646-4380353 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6