Detailed information of TA system TA101630

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2613571..2613796
• Replicon: chromosome
• Accession: NZ_CP028596
• Organism: Escherichia coli strain 149

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: C9E71_RS13565
• Protein ID: WP_000813258.1
• Coordinates: 2613641..2613796 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2613571..2613629 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E71_RS13515	2608636..2608878	+	243	WP_000747948.1	hypothetical protein	-
C9E71_RS13520	2608862..2609287	+	426	WP_000693878.1	Rha family transcriptional regulator	-
C9E71_RS13525	2609356..2610399	+	1044	WP_001262402.1	hypothetical protein	-
C9E71_RS13530	2610392..2610853	+	462	WP_000139447.1	replication protein	-
C9E71_RS13535	2610887..2611603	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
C9E71_RS13540	2611636..2611917	+	282	WP_000603384.1	DNA-binding protein	-
C9E71_RS13545	2611914..2612141	+	228	WP_000699808.1	hypothetical protein	-
C9E71_RS13550	2612134..2612445	+	312	WP_001289673.1	hypothetical protein	-
C9E71_RS13555	2612573..2612791	+	219	WP_000683609.1	DUF4014 family protein	-
C9E71_RS13560	2612793..2613350	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2613571..2613629	-	59	-	-	Antitoxin
C9E71_RS13565	2613641..2613796	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
C9E71_RS13570	2613916..2614260	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
C9E71_RS13575	2614382..2614654	+	273	WP_000191872.1	hypothetical protein	-
C9E71_RS13580	2614656..2615705	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
C9E71_RS13585	2615718..2616023	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
C9E71_RS13590	2616086..2616640	+	555	WP_000640035.1	DUF1133 family protein	-
C9E71_RS13595	2616865..2617062	+	198	WP_000917763.1	hypothetical protein	-
C9E71_RS13600	2617198..2617911	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
C9E71_RS13615	2618362..2618793	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2551778..2644247	92469

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T101630 WP_000813258.1 NZ_CP028596:2613641-2613796 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T101630 NZ_CP028596:2613641-2613796 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT101630 NZ_CP028596:c2613629-2613571 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin