Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/- |
| Location | 1002360..1002930 | Replicon | chromosome |
| Accession | NC_004757 | ||
| Organism | Nitrosomonas europaea ATCC 19718 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | Q82VX8 |
| Locus tag | ALW85_RS04820 | Protein ID | WP_011111532.1 |
| Coordinates | 1002360..1002710 (-) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | A0A837X5V9 |
| Locus tag | ALW85_RS04825 | Protein ID | WP_041356592.1 |
| Coordinates | 1002700..1002930 (-) | Length | 77 a.a. |
Genomic Context
Location: 997469..997969 (501 bp)
Type: Others
Protein ID: WP_011111527.1
Type: Others
Protein ID: WP_011111527.1
Location: 998204..998779 (576 bp)
Type: Others
Protein ID: WP_041356590.1
Type: Others
Protein ID: WP_041356590.1
Location: 998776..999465 (690 bp)
Type: Others
Protein ID: WP_011111529.1
Type: Others
Protein ID: WP_011111529.1
Location: 999579..1001864 (2286 bp)
Type: Others
Protein ID: WP_011111530.1
Type: Others
Protein ID: WP_011111530.1
Location: 1001861..1002328 (468 bp)
Type: Others
Protein ID: WP_011111531.1
Type: Others
Protein ID: WP_011111531.1
Location: 1003119..1005425 (2307 bp)
Type: Others
Protein ID: WP_011111533.1
Type: Others
Protein ID: WP_011111533.1
Location: 1002360..1002710 (351 bp)
Type: Toxin
Protein ID: WP_011111532.1
Type: Toxin
Protein ID: WP_011111532.1
Location: 1002700..1002930 (231 bp)
Type: Antitoxin
Protein ID: WP_041356592.1
Type: Antitoxin
Protein ID: WP_041356592.1
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Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ALW85_RS04795 (NE0916) | 997469..997969 | + | 501 | WP_011111527.1 | TPM domain-containing protein | - |
| ALW85_RS04800 (NE0917) | 998204..998779 | + | 576 | WP_041356590.1 | RNA polymerase sigma factor | - |
| ALW85_RS04805 (NE0918) | 998776..999465 | + | 690 | WP_011111529.1 | DUF3619 family protein | - |
| ALW85_RS04810 (NE0919) | 999579..1001864 | + | 2286 | WP_011111530.1 | ABC transporter ATP-binding protein/permease | - |
| ALW85_RS04815 (NE0920) | 1001861..1002328 | + | 468 | WP_011111531.1 | DUF1854 domain-containing protein | - |
| ALW85_RS04820 (NE0921) | 1002360..1002710 | - | 351 | WP_011111532.1 | type II toxin-antitoxin system PemK/MazF family toxin | Toxin |
| ALW85_RS04825 (RK28_RS04815) | 1002700..1002930 | - | 231 | WP_041356592.1 | type II toxin-antitoxin system PrlF family antitoxin | Antitoxin |
| ALW85_RS04830 (NE0922) | 1003119..1005425 | + | 2307 | WP_011111533.1 | cyanophycin synthetase | - |
Associated MGEs
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MGE detail | Similar MGEs | Relative position | MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| No matching records found | |||||||
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12670.73 Da Isoelectric Point: 9.0855
>T10162 WP_011111532.1 NC_004757:c1002710-1002360 [Nitrosomonas europaea ATCC 19718]
VSYERFTVLKVPFPFTDRTAAKNRPALVLSDAATFNDPIGHSVLAMITSAANPAWPLDCLIDDLVSAGLPAPSVVRFKLF
TLDHRLIRGELGRLAVSDSIQVTRSLYQLFGMAAVR
VSYERFTVLKVPFPFTDRTAAKNRPALVLSDAATFNDPIGHSVLAMITSAANPAWPLDCLIDDLVSAGLPAPSVVRFKLF
TLDHRLIRGELGRLAVSDSIQVTRSLYQLFGMAAVR
Download Length: 351 bp
>T10162 NC_004757:c1002710-1002360 [Nitrosomonas europaea ATCC 19718]
GTGAGTTATGAACGTTTCACGGTATTGAAAGTGCCTTTTCCGTTTACCGACCGTACGGCTGCCAAAAACCGTCCGGCACT
CGTACTCTCGGATGCAGCCACATTCAACGATCCAATCGGCCATTCGGTGCTGGCCATGATTACCTCAGCCGCCAATCCAG
CCTGGCCGCTCGATTGCCTGATCGATGATCTGGTCAGTGCCGGTCTCCCGGCTCCTTCTGTCGTACGCTTCAAGCTGTTC
ACGCTCGATCACCGGCTGATACGTGGTGAACTTGGACGACTTGCCGTCAGTGATAGTATTCAGGTCACACGATCTCTGTA
TCAGCTTTTCGGAATGGCTGCAGTGCGATAA
GTGAGTTATGAACGTTTCACGGTATTGAAAGTGCCTTTTCCGTTTACCGACCGTACGGCTGCCAAAAACCGTCCGGCACT
CGTACTCTCGGATGCAGCCACATTCAACGATCCAATCGGCCATTCGGTGCTGGCCATGATTACCTCAGCCGCCAATCCAG
CCTGGCCGCTCGATTGCCTGATCGATGATCTGGTCAGTGCCGGTCTCCCGGCTCCTTCTGTCGTACGCTTCAAGCTGTTC
ACGCTCGATCACCGGCTGATACGTGGTGAACTTGGACGACTTGCCGTCAGTGATAGTATTCAGGTCACACGATCTCTGTA
TCAGCTTTTCGGAATGGCTGCAGTGCGATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8415.63 Da Isoelectric Point: 4.5557
>AT10162 WP_041356592.1 NC_004757:c1002930-1002700 [Nitrosomonas europaea ATCC 19718]
MTIIAKVTSKGQTTIPADIRAALRIKPGDLIIWEMSDDGSARIRRVQPLDIEYLKAVEGTLSEWAGAADEEAYREL
MTIIAKVTSKGQTTIPADIRAALRIKPGDLIIWEMSDDGSARIRRVQPLDIEYLKAVEGTLSEWAGAADEEAYREL
Download Length: 231 bp
>AT10162 NC_004757:c1002930-1002700 [Nitrosomonas europaea ATCC 19718]
ATGACTATTATTGCAAAAGTAACCTCAAAGGGACAGACCACGATACCGGCTGATATTCGTGCAGCGTTGCGTATAAAACC
GGGCGATTTGATTATCTGGGAAATGTCGGATGATGGTAGTGCACGCATTCGACGTGTACAACCGCTCGATATCGAATACT
TGAAAGCAGTGGAGGGAACGCTTTCCGAGTGGGCAGGCGCAGCCGATGAGGAAGCTTATCGTGAGTTATGA
ATGACTATTATTGCAAAAGTAACCTCAAAGGGACAGACCACGATACCGGCTGATATTCGTGCAGCGTTGCGTATAAAACC
GGGCGATTTGATTATCTGGGAAATGTCGGATGATGGTAGTGCACGCATTCGACGTGTACAACCGCTCGATATCGAATACT
TGAAAGCAGTGGAGGGAACGCTTTCCGAGTGGGCAGGCGCAGCCGATGAGGAAGCTTATCGTGAGTTATGA
Similar Proteins
Only experimentally validated proteins are listed.
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Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6259 | Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 | 30.973 | 97.414 | 0.302 |
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Multiple sequence alignment
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Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| No matching records found | ||||
Structures
Toxin
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Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q82VX8 |
Antitoxin
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Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A837X5V9 |
References
(1) Tatsuki Miyamoto et al. (2018) Nitrosomonas europaea MazF Specifically Recognises the UGG Motif and Promotes Selective RNA Degradation. Frontiers in Microbiology 9:2386. [PubMed:30349517]