Detailed information of TA system TA010162

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/-
• Location: 1002360..1002930
• Replicon: chromosome
• Accession: NC_004757
• Organism: Nitrosomonas europaea ATCC 19718

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q82VX8
• Locus tag: ALW85_RS04820
• Protein ID: WP_011111532.1
• Coordinates: 1002360..1002710 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A837X5V9
• Locus tag: ALW85_RS04825
• Protein ID: WP_041356592.1
• Coordinates: 1002700..1002930 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALW85_RS04795 (NE0916)	997469..997969	+	501	WP_011111527.1	TPM domain-containing protein	-
ALW85_RS04800 (NE0917)	998204..998779	+	576	WP_041356590.1	RNA polymerase sigma factor	-
ALW85_RS04805 (NE0918)	998776..999465	+	690	WP_011111529.1	DUF3619 family protein	-
ALW85_RS04810 (NE0919)	999579..1001864	+	2286	WP_011111530.1	ABC transporter ATP-binding protein/permease	-
ALW85_RS04815 (NE0920)	1001861..1002328	+	468	WP_011111531.1	DUF1854 domain-containing protein	-
ALW85_RS04820 (NE0921)	1002360..1002710	-	351	WP_011111532.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ALW85_RS04825 (RK28_RS04815)	1002700..1002930	-	231	WP_041356592.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
ALW85_RS04830 (NE0922)	1003119..1005425	+	2307	WP_011111533.1	cyanophycin synthetase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-109)

Antitoxin

• PrlF antitoxin (5-47)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12670.73 Da
• Isoelectric Point: 9.0855

>T10162 WP_011111532.1 NC_004757:c1002710-1002360 [Nitrosomonas europaea ATCC 19718]
VSYERFTVLKVPFPFTDRTAAKNRPALVLSDAATFNDPIGHSVLAMITSAANPAWPLDCLIDDLVSAGLPAPSVVRFKLF
TLDHRLIRGELGRLAVSDSIQVTRSLYQLFGMAAVR

Nucleotide

• Length: 351 bp

>T10162 NC_004757:c1002710-1002360 [Nitrosomonas europaea ATCC 19718]
GTGAGTTATGAACGTTTCACGGTATTGAAAGTGCCTTTTCCGTTTACCGACCGTACGGCTGCCAAAAACCGTCCGGCACT
CGTACTCTCGGATGCAGCCACATTCAACGATCCAATCGGCCATTCGGTGCTGGCCATGATTACCTCAGCCGCCAATCCAG
CCTGGCCGCTCGATTGCCTGATCGATGATCTGGTCAGTGCCGGTCTCCCGGCTCCTTCTGTCGTACGCTTCAAGCTGTTC
ACGCTCGATCACCGGCTGATACGTGGTGAACTTGGACGACTTGCCGTCAGTGATAGTATTCAGGTCACACGATCTCTGTA
TCAGCTTTTCGGAATGGCTGCAGTGCGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8415.63 Da
• Isoelectric Point: 4.5557

>AT10162 WP_041356592.1 NC_004757:c1002930-1002700 [Nitrosomonas europaea ATCC 19718]
MTIIAKVTSKGQTTIPADIRAALRIKPGDLIIWEMSDDGSARIRRVQPLDIEYLKAVEGTLSEWAGAADEEAYREL

Nucleotide

• Length: 231 bp

>AT10162 NC_004757:c1002930-1002700 [Nitrosomonas europaea ATCC 19718]
ATGACTATTATTGCAAAAGTAACCTCAAAGGGACAGACCACGATACCGGCTGATATTCGTGCAGCGTTGCGTATAAAACC
GGGCGATTTGATTATCTGGGAAATGTCGGATGATGGTAGTGCACGCATTCGACGTGTACAACCGCTCGATATCGAATACT
TGAAAGCAGTGGAGGGAACGCTTTCCGAGTGGGCAGGCGCAGCCGATGAGGAAGCTTATCGTGAGTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T6259	Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222	30.973	97.414	0.302

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: Q82VX8

Antitoxin

• AlphaFold DB: A0A837X5V9

References

(1) Tatsuki Miyamoto et al. (2018) Nitrosomonas europaea MazF Specifically Recognises the UGG Motif and Promotes Selective RNA Degradation. Frontiers in Microbiology 9:2386. [PubMed:30349517]


 experimental literature