Detailed information of TA system TA101601

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4520024..4520718
• Replicon: chromosome
• Accession: NZ_CP028592
• Organism: Escherichia coli strain 150

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: C9E72_RS23635
• Protein ID: WP_001263495.1
• Coordinates: 4520024..4520422 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: C9E72_RS23640
• Protein ID: WP_000554757.1
• Coordinates: 4520425..4520718 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	4515611..4515691	-	81	NuclAT_11	-	-
-	4515611..4515691	-	81	NuclAT_11	-	-
-	4515611..4515691	-	81	NuclAT_11	-	-
-	4515611..4515691	-	81	NuclAT_11	-	-
C9E72_RS23610	4516287..4516745	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
C9E72_RS23615	4517006..4518463	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
C9E72_RS23620	4518520..4519041	-	522	Protein_4492	peptide chain release factor H	-
C9E72_RS23625	4519040..4519243	-	204	Protein_4493	RNA ligase RtcB family protein	-
C9E72_RS23630	4519562..4520014	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
C9E72_RS23635	4520024..4520422	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C9E72_RS23640	4520425..4520718	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C9E72_RS23645	4520770..4521825	-	1056	WP_001226186.1	DNA polymerase IV	-
C9E72_RS23650	4521896..4522681	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
C9E72_RS23655	4522653..4524365	+	1713	Protein_4499	flagellar biosynthesis protein FlhA	-
C9E72_RS23660	4524510..4525007	-	498	Protein_4500	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T101601 WP_001263495.1 NZ_CP028592:c4520422-4520024 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T101601 NZ_CP028592:c4520422-4520024 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT101601 WP_000554757.1 NZ_CP028592:c4520718-4520425 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT101601 NZ_CP028592:c4520718-4520425 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6