Detailed information of TA system TA101592

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2697291..2697516
• Replicon: chromosome
• Accession: NZ_CP028592
• Organism: Escherichia coli strain 150

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: C9E72_RS14120
• Protein ID: WP_000813258.1
• Coordinates: 2697361..2697516 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2697291..2697349 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E72_RS14070	2692356..2692598	+	243	WP_000747948.1	hypothetical protein	-
C9E72_RS14075	2692582..2693007	+	426	WP_000693878.1	Rha family transcriptional regulator	-
C9E72_RS14080	2693076..2694119	+	1044	WP_001262402.1	hypothetical protein	-
C9E72_RS14085	2694112..2694573	+	462	WP_000139447.1	replication protein	-
C9E72_RS14090	2694607..2695323	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
C9E72_RS14095	2695356..2695637	+	282	WP_000603384.1	DNA-binding protein	-
C9E72_RS14100	2695634..2695861	+	228	WP_000699809.1	hypothetical protein	-
C9E72_RS14105	2695854..2696165	+	312	WP_001289673.1	hypothetical protein	-
C9E72_RS14110	2696293..2696511	+	219	WP_137049466.1	DUF4014 family protein	-
C9E72_RS14115	2696513..2697070	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	2697291..2697349	-	59	-	-	Antitoxin
C9E72_RS14120	2697361..2697516	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
C9E72_RS14125	2697636..2697980	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
C9E72_RS14130	2698102..2698374	+	273	WP_000191872.1	hypothetical protein	-
C9E72_RS14135	2698376..2699425	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
C9E72_RS14140	2699438..2699743	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
C9E72_RS14145	2699806..2700360	+	555	WP_000640035.1	DUF1133 family protein	-
C9E72_RS14150	2700585..2700782	+	198	WP_000917763.1	hypothetical protein	-
C9E72_RS14155	2700919..2701632	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
C9E72_RS14170	2702087..2702515	+	429	WP_001303509.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1 / nleG7'	2571722..2734632	162910

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T101592 WP_000813258.1 NZ_CP028592:2697361-2697516 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T101592 NZ_CP028592:2697361-2697516 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT101592 NZ_CP028592:c2697349-2697291 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin