Detailed information of TA system TA101590

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2649216..2649441
• Replicon: chromosome
• Accession: NZ_CP028592
• Organism: Escherichia coli strain 150

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: C9E72_RS13805
• Protein ID: WP_000813254.1
• Coordinates: 2649286..2649441 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2649216..2649274 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C9E72_RS13760	2644469..2645422	+	954	WP_000095667.1	helix-turn-helix domain-containing protein	-
C9E72_RS13765	2645429..2646169	+	741	WP_000790456.1	ATP-binding protein	-
C9E72_RS13770	2646199..2646969	+	771	WP_000450888.1	DUF1627 domain-containing protein	-
C9E72_RS13775	2646985..2647380	+	396	WP_001118161.1	DUF977 family protein	-
C9E72_RS13780	2647437..2647793	+	357	WP_001302146.1	hypothetical protein	-
C9E72_RS13785	2647842..2648054	+	213	WP_000063625.1	hypothetical protein	-
C9E72_RS13790	2648090..2648461	+	372	WP_000137941.1	hypothetical protein	-
C9E72_RS13795	2648458..2648820	+	363	WP_000610379.1	DUF551 domain-containing protein	-
C9E72_RS13800	2648936..2649040	+	105	WP_001278450.1	hypothetical protein	-
-	2649216..2649274	-	59	-	-	Antitoxin
C9E72_RS13805	2649286..2649441	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
C9E72_RS29435	2649738..2649905	+	168	WP_000998188.1	hypothetical protein	-
C9E72_RS13810	2649971..2650249	+	279	WP_001304183.1	hypothetical protein	-
C9E72_RS13815	2650251..2651300	+	1050	Protein_2635	DUF968 domain-containing protein	-
C9E72_RS13820	2651313..2651687	+	375	WP_000904137.1	RusA family crossover junction endodeoxyribonuclease	-
C9E72_RS13825	2651684..2652505	+	822	WP_000762904.1	antitermination protein	-
C9E72_RS13830	2652732..2652929	+	198	WP_000917735.1	hypothetical protein	-
C9E72_RS13835	2653080..2654138	+	1059	WP_000483497.1	site-specific DNA-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1 / nleG7'	2571722..2734632	162910

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T101590 WP_000813254.1 NZ_CP028592:2649286-2649441 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T101590 NZ_CP028592:2649286-2649441 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGCTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT101590 NZ_CP028592:c2649274-2649216 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACGTGTGTAGAGCATAGATATGGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin