Detailed information of TA system TA101572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4895075..4895677
• Replicon: chromosome
• Accession: NZ_CP028589
• Organism: Escherichia coli strain WCHEC4533

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: DBR03_RS25895
• Protein ID: WP_000897305.1
• Coordinates: 4895366..4895677 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DBR03_RS25890
• Protein ID: WP_000356395.1
• Coordinates: 4895075..4895365 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR03_RS25850	4890698..4891600	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
DBR03_RS25855	4891597..4892232	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
DBR03_RS25860	4892229..4893158	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
DBR03_RS25865	4893340..4893582	-	243	WP_021523315.1	hypothetical protein	-
DBR03_RS25870	4893801..4894019	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
DBR03_RS25880	4894438..4894716	-	279	WP_001306650.1	hypothetical protein	-
DBR03_RS25890	4895075..4895365	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
DBR03_RS25895	4895366..4895677	-	312	WP_000897305.1	hypothetical protein	Toxin
DBR03_RS25900	4895906..4896814	+	909	WP_001306651.1	alpha/beta hydrolase	-
DBR03_RS25905	4896983..4898797	-	1815	WP_020233236.1	hypothetical protein	-
DBR03_RS25910	4899213..4900154	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
DBR03_RS25915	4900199..4900636	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T101572 WP_000897305.1 NZ_CP028589:c4895677-4895366 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T101572 NZ_CP028589:c4895677-4895366 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT101572 WP_000356395.1 NZ_CP028589:c4895365-4895075 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT101572 NZ_CP028589:c4895365-4895075 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7