Detailed information of TA system TA101565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3938383..3939077
• Replicon: chromosome
• Accession: NZ_CP028589
• Organism: Escherichia coli strain WCHEC4533

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: DBR03_RS21240
• Protein ID: WP_001521903.1
• Coordinates: 3938383..3938781 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: DBR03_RS21245
• Protein ID: WP_000554758.1
• Coordinates: 3938784..3939077 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR03_RS21210	3933553..3934797	-	1245	WP_000189541.1	esterase FrsA	-
-	3934213..3934293	-	81	NuclAT_10	-	-
-	3934213..3934293	-	81	NuclAT_10	-	-
-	3934213..3934293	-	81	NuclAT_10	-	-
-	3934213..3934293	-	81	NuclAT_10	-	-
DBR03_RS21215	3934889..3935347	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
DBR03_RS21220	3935608..3937065	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
DBR03_RS27090	3937122..3937262	-	141	WP_166739706.1	peptide chain release factor	-
DBR03_RS21230	3937470..3937676	-	207	Protein_3721	RtcB family protein	-
DBR03_RS21235	3937921..3938373	-	453	WP_023144376.1	GNAT family N-acetyltransferase	-
DBR03_RS21240	3938383..3938781	-	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DBR03_RS21245	3938784..3939077	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DBR03_RS21250	3939129..3940184	-	1056	WP_001226177.1	DNA polymerase IV	-
DBR03_RS21255	3940255..3941040	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
DBR03_RS21260	3940985..3942724	+	1740	WP_021523009.1	flagellar biosynthesis protein FlhA	-
DBR03_RS21265	3942803..3943156	+	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
DBR03_RS21270	3943213..3943962	-	750	WP_016233951.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3928320..3939077	10757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T101565 WP_001521903.1 NZ_CP028589:c3938781-3938383 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T101565 NZ_CP028589:c3938781-3938383 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT101565 WP_000554758.1 NZ_CP028589:c3939077-3938784 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT101565 NZ_CP028589:c3939077-3938784 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3