Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1944331..1944833 Replicon chromosome
Accession NZ_CP028589
Organism Escherichia coli strain WCHEC4533

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DBR03_RS11325 Protein ID WP_000767829.1
Coordinates 1944579..1944833 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DBR03_RS11320 Protein ID WP_001259255.1
Coordinates 1944331..1944582 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBR03_RS11295 1939509..1940576 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
DBR03_RS11300 1940576..1941646 - 1071 WP_000108937.1 histidinol-phosphate transaminase -
DBR03_RS11305 1941643..1942947 - 1305 WP_021523144.1 histidinol dehydrogenase -
DBR03_RS11310 1942953..1943852 - 900 WP_021523143.1 ATP phosphoribosyltransferase -
DBR03_RS11315 1943998..1944048 - 51 WP_001364200.1 his operon leader peptide -
DBR03_RS11320 1944331..1944582 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DBR03_RS11325 1944579..1944833 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DBR03_RS11330 1944916..1945740 + 825 WP_000754758.1 SDR family oxidoreductase -
DBR03_RS11335 1945786..1946715 + 930 WP_023154044.1 LysR family transcriptional regulator -
DBR03_RS11340 1946930..1946992 + 63 WP_010723108.1 membrane protein YoeI -
DBR03_RS11345 1946982..1948340 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DBR03_RS11350 1948519..1949577 + 1059 WP_000492324.1 transport protein YeeE -
DBR03_RS11355 1949591..1949818 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T101549 WP_000767829.1 NZ_CP028589:1944579-1944833 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T101549 NZ_CP028589:1944579-1944833 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT101549 WP_001259255.1 NZ_CP028589:1944331-1944582 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT101549 NZ_CP028589:1944331-1944582 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References