101549

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1944331..1944833 Replicon chromosome
Accession NZ_CP028589
Organism Escherichia coli strain WCHEC4533

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag DBR03_RS11325 Protein ID WP_000767829.1
Coordinates 1944579..1944833 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag DBR03_RS11320 Protein ID WP_001259255.1
Coordinates 1944331..1944582 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBR03_RS11295 1939509..1940576 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
DBR03_RS11300 1940576..1941646 - 1071 WP_000108937.1 histidinol-phosphate transaminase -
DBR03_RS11305 1941643..1942947 - 1305 WP_021523144.1 histidinol dehydrogenase -
DBR03_RS11310 1942953..1943852 - 900 WP_021523143.1 ATP phosphoribosyltransferase -
DBR03_RS11315 1943998..1944048 - 51 WP_001364200.1 his operon leader peptide -
DBR03_RS11320 1944331..1944582 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DBR03_RS11325 1944579..1944833 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
DBR03_RS11330 1944916..1945740 + 825 WP_000754758.1 SDR family oxidoreductase -
DBR03_RS11335 1945786..1946715 + 930 WP_023154044.1 LysR family transcriptional regulator -
DBR03_RS11340 1946930..1946992 + 63 WP_010723108.1 membrane protein YoeI -
DBR03_RS11345 1946982..1948340 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
DBR03_RS11350 1948519..1949577 + 1059 WP_000492324.1 transport protein YeeE -
DBR03_RS11355 1949591..1949818 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T101549 WP_000767829.1 NZ_CP028589:1944579-1944833 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T101549 NZ_CP028589:1944579-1944833 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT101549 WP_001259255.1 NZ_CP028589:1944331-1944582 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT101549 NZ_CP028589:1944331-1944582 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References