Detailed information of TA system TA101534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 82865..83576
• Replicon: plasmid p1_000533
• Accession: NZ_CP028585
• Organism: Escherichia coli strain WCHEC4533

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: DBR03_RS00575
• Protein ID: WP_000162415.1
• Coordinates: 83274..83576 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DBR03_RS00570
• Protein ID: WP_000806445.1
• Coordinates: 82865..83203 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR03_RS00535	78655..79308	+	654	WP_000410954.1	hypothetical protein	-
DBR03_RS00540	79645..79926	+	282	WP_032323275.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DBR03_RS00545	79935..80216	+	282	WP_032323276.1	HigA family addiction module antidote protein	-
DBR03_RS00550	80309..80638	+	330	WP_000542383.1	hypothetical protein	-
DBR03_RS00555	80631..81824	+	1194	WP_063074560.1	hypothetical protein	-
DBR03_RS00560	81858..82586	-	729	WP_080077649.1	hypothetical protein	-
DBR03_RS00565	82608..82808	-	201	WP_001022420.1	hypothetical protein	-
DBR03_RS00570	82865..83203	-	339	WP_000806445.1	HigA family addiction module antidote protein	Antitoxin
DBR03_RS00575	83274..83576	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DBR03_RS00580	83739..84530	+	792	WP_063074813.1	hypothetical protein	-
DBR03_RS00585	84527..85294	+	768	WP_000203293.1	hypothetical protein	-
DBR03_RS00590	85298..86278	+	981	WP_069936439.1	tail length tape measure protein	-
DBR03_RS00595	86275..86928	+	654	WP_087503501.1	baseplate protein	-
DBR03_RS00600	86988..87893	+	906	WP_087503502.1	recombination-associated protein RdgC	-
DBR03_RS00605	87877..88557	+	681	WP_000812238.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92537	92537

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T101534 WP_000162415.1 NZ_CP028585:c83576-83274 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T101534 NZ_CP028585:c83576-83274 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT101534 WP_000806445.1 NZ_CP028585:c83203-82865 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT101534 NZ_CP028585:c83203-82865 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTTCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3