Detailed information of TA system TA101533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 79645..80216
• Replicon: plasmid p1_000533
• Accession: NZ_CP028585
• Organism: Escherichia coli strain WCHEC4533

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DBR03_RS00540
• Protein ID: WP_032323275.1
• Coordinates: 79645..79926 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DBR03_RS00545
• Protein ID: WP_032323276.1
• Coordinates: 79935..80216 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBR03_RS26910	75362..75529	+	168	WP_162983138.1	hypothetical protein	-
DBR03_RS00505	75703..75822	+	120	WP_071526433.1	ash family protein	-
DBR03_RS00510	75841..76056	+	216	WP_022630900.1	host cell division inhibitor Icd-like protein	-
DBR03_RS00515	76056..76982	+	927	WP_072275558.1	Rha family transcriptional regulator	-
DBR03_RS00520	77042..77353	+	312	WP_000717724.1	hypothetical protein	-
DBR03_RS00525	77350..77577	-	228	WP_001396850.1	hypothetical protein	-
DBR03_RS00530	77763..78371	-	609	WP_032323273.1	hypothetical protein	-
DBR03_RS00535	78655..79308	+	654	WP_000410954.1	hypothetical protein	-
DBR03_RS00540	79645..79926	+	282	WP_032323275.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DBR03_RS00545	79935..80216	+	282	WP_032323276.1	HigA family addiction module antidote protein	Antitoxin
DBR03_RS00550	80309..80638	+	330	WP_000542383.1	hypothetical protein	-
DBR03_RS00555	80631..81824	+	1194	WP_063074560.1	hypothetical protein	-
DBR03_RS00560	81858..82586	-	729	WP_080077649.1	hypothetical protein	-
DBR03_RS00565	82608..82808	-	201	WP_001022420.1	hypothetical protein	-
DBR03_RS00570	82865..83203	-	339	WP_000806445.1	HigA family addiction module antidote protein	-
DBR03_RS00575	83274..83576	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DBR03_RS00580	83739..84530	+	792	WP_063074813.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92537	92537

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-86)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10606.14 Da
• Isoelectric Point: 10.2589

>T101533 WP_032323275.1 NZ_CP028585:79645-79926 [Escherichia coli]
MIKSFRHKGLERFFKTGSTAGIQAKHAVKLRIQLTALNTAKRPDDMSAPGWELHPLKGADLKGHWAISVNGNWRLTFRFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T101533 NZ_CP028585:79645-79926 [Escherichia coli]
TTGATTAAGTCATTTCGCCATAAAGGGCTTGAACGCTTTTTCAAGACAGGTTCAACCGCAGGCATTCAGGCCAAACATGC
GGTTAAGCTACGCATTCAATTGACTGCGCTTAATACAGCTAAACGCCCGGACGATATGTCTGCGCCTGGATGGGAATTGC
ACCCTCTAAAGGGGGCGGACCTTAAAGGTCACTGGGCAATATCGGTAAACGGTAACTGGCGACTTACCTTCCGATTTGAA
GGAGAAGACGCAATTCTAGTTGACTATCAGGATTATCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10160.63 Da
• Isoelectric Point: 7.3179

>AT101533 WP_032323276.1 NZ_CP028585:79935-80216 [Escherichia coli]
MSRMHNPAHPGAVLREYLGDMSVTDAAAALGITRTALSRILNEKAGISADMALRLESALGTSAEMWTGMQSQYELWVASQ
TTRPNIRPLFFHA

Nucleotide

• Length: 282 bp

>AT101533 NZ_CP028585:79935-80216 [Escherichia coli]
ATGAGTCGTATGCATAATCCCGCGCATCCAGGCGCGGTTCTTCGTGAATATTTGGGAGACATGTCAGTCACAGACGCCGC
TGCGGCATTAGGCATTACTCGCACGGCGCTTTCCCGAATTCTGAATGAAAAAGCAGGTATATCTGCTGATATGGCACTTC
GTCTGGAGTCCGCTCTTGGAACCAGTGCCGAAATGTGGACCGGTATGCAATCCCAGTATGAGCTTTGGGTTGCTTCGCAA
ACCACTCGCCCAAACATCCGTCCATTATTTTTCCACGCATAA