Detailed information of TA system TA101531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5852461..5853056
• Replicon: chromosome
• Accession: NZ_CP028584
• Organism: Pseudomonas aeruginosa strain WCHPA075019

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DBA97_RS27585
• Protein ID: WP_079752024.1
• Coordinates: 5852778..5853056 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DBA97_RS27580
• Protein ID: WP_083556829.1
• Coordinates: 5852461..5852766 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBA97_RS27565	5849185..5850048	-	864	WP_023098850.1	integrase domain-containing protein	-
DBA97_RS27570	5850649..5851791	-	1143	WP_023098851.1	hypothetical protein	-
DBA97_RS27580	5852461..5852766	-	306	WP_083556829.1	HigA family addiction module antidote protein	Antitoxin
DBA97_RS27585	5852778..5853056	-	279	WP_079752024.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DBA97_RS27590	5853109..5853335	-	227	Protein_5330	tyrosine-type recombinase/integrase	-
DBA97_RS27595	5853704..5853907	+	204	WP_033971061.1	hypothetical protein	-
DBA97_RS27605	5854329..5854844	-	516	WP_033971063.1	hypothetical protein	-
DBA97_RS27610	5855151..5855801	-	651	WP_033971066.1	carbonate dehydratase	-
DBA97_RS27615	5855863..5857101	-	1239	WP_088376614.1	C69 family dipeptidase	-
DBA97_RS27620	5857300..5857752	-	453	WP_088376613.1	ribosomal protein S18-alanine N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5828905..5853907	25002

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10673.26 Da
• Isoelectric Point: 8.5538

>T101531 WP_079752024.1 NZ_CP028584:c5853056-5852778 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAAKELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T101531 NZ_CP028584:c5853056-5852778 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAAGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11243.78 Da
• Isoelectric Point: 4.7860

>AT101531 WP_083556829.1 NZ_CP028584:c5852766-5852461 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELKISPTALARALNVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT101531 NZ_CP028584:c5852766-5852461 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGAAGATCTCTCC
AACTGCTCTAGCACGCGCTTTGAACGTCTCCGCCCCGACAGTGAACGATATTGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGCCTGGGGCGTTACTTTGACACGTCCGCTCAGTTCTGGATGAATCTTCAGAGTGAGTATTCGTTGGCA
ACTGCTTATGCGGCGAACGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG