Detailed information of TA system TA101529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5098897..5099578
• Replicon: chromosome
• Accession: NZ_CP028584
• Organism: Pseudomonas aeruginosa strain WCHPA075019

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DBA97_RS24060
• Protein ID: WP_015503432.1
• Coordinates: 5099213..5099578 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DBA97_RS24055
• Protein ID: WP_016561576.1
• Coordinates: 5098897..5099220 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DBA97_RS24025	5094518..5095396	+	879	WP_003109435.1	hypothetical protein	-
DBA97_RS32535	5095908..5096774	-	867	WP_023081971.1	hypothetical protein	-
DBA97_RS32540	5096804..5097055	-	252	WP_124136459.1	hypothetical protein	-
DBA97_RS24035	5097272..5097826	-	555	WP_074197746.1	tyrosine-type recombinase/integrase	-
DBA97_RS24040	5098000..5098275	-	276	WP_004353146.1	hypothetical protein	-
DBA97_RS24045	5098275..5098607	-	333	WP_088376638.1	hypothetical protein	-
DBA97_RS24050	5098662..5098781	+	120	Protein_4645	IS5/IS1182 family transposase	-
DBA97_RS24055	5098897..5099220	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
DBA97_RS24060	5099213..5099578	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DBA97_RS24065	5099842..5100084	-	243	WP_043884955.1	hypothetical protein	-
DBA97_RS24070	5100291..5100563	+	273	WP_003085667.1	hypothetical protein	-
DBA97_RS24075	5100582..5101007	-	426	WP_003163196.1	VOC family protein	-
DBA97_RS24080	5101108..5101992	+	885	WP_019725847.1	LysR family transcriptional regulator	-
DBA97_RS24085	5101965..5102918	-	954	WP_003085661.1	LysR family transcriptional regulator	-
DBA97_RS24090	5103139..5103573	+	435	WP_003158601.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T101529 WP_015503432.1 NZ_CP028584:c5099578-5099213 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T101529 NZ_CP028584:c5099578-5099213 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT101529 WP_016561576.1 NZ_CP028584:c5099220-5098897 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT101529 NZ_CP028584:c5099220-5098897 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA