Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2535713..2536215 Replicon chromosome
Accession NZ_CP028584
Organism Pseudomonas aeruginosa strain WCHPA075019

Toxin (Protein)


Gene name yoeB Uniprot ID A0A1C7CBF7
Locus tag DBA97_RS12020 Protein ID WP_023081358.1
Coordinates 2535961..2536215 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A1C7C600
Locus tag DBA97_RS12015 Protein ID WP_004350479.1
Coordinates 2535713..2535964 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBA97_RS11980 2531514..2531996 + 483 WP_003090905.1 GAF domain-containing protein -
DBA97_RS11985 2532006..2532668 - 663 WP_003114770.1 glutathione S-transferase family protein -
DBA97_RS11990 2532730..2533503 - 774 WP_003090903.1 hypothetical protein -
DBA97_RS12010 2534258..2535628 + 1371 WP_023081359.1 tyrosine-type recombinase/integrase -
DBA97_RS12015 2535713..2535964 + 252 WP_004350479.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
DBA97_RS12020 2535961..2536215 + 255 WP_023081358.1 Txe/YoeB family addiction module toxin Toxin
DBA97_RS12025 2536441..2536836 + 396 WP_023081357.1 DUF3742 family protein -
DBA97_RS12030 2536844..2538364 - 1521 WP_004350477.1 conjugal transfer protein TraG N-terminal domain-containing protein -
DBA97_RS12035 2538379..2538729 - 351 WP_023081356.1 hypothetical protein -
DBA97_RS12040 2538917..2539780 - 864 WP_004350470.1 integrase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10061.50 Da        Isoelectric Point: 8.4789

>T101526 WP_023081358.1 NZ_CP028584:2535961-2536215 [Pseudomonas aeruginosa]
VKLIWSEQAWDDYLYWQDTDKRMVKKVNELIKDACRTPFEGKGKPEPLRHNLAGFWSRRITAEHRFVYSVTDDALLIAAC
RYHY

Download         Length: 255 bp

>T101526 NZ_CP028584:2535961-2536215 [Pseudomonas aeruginosa]
GTGAAGCTGATCTGGTCGGAACAAGCCTGGGACGATTATCTGTACTGGCAGGACACGGATAAGCGTATGGTGAAAAAGGT
CAACGAGTTGATCAAGGATGCCTGCCGTACCCCGTTCGAGGGCAAGGGCAAGCCGGAGCCGCTTCGCCACAACCTGGCGG
GCTTCTGGTCGAGACGGATCACGGCCGAGCATCGGTTTGTGTATTCGGTGACGGATGACGCGCTGCTGATTGCAGCCTGC
CGTTACCACTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9363.65 Da        Isoelectric Point: 4.5843

>AT101526 WP_004350479.1 NZ_CP028584:2535713-2535964 [Pseudomonas aeruginosa]
MRTISYTEARQNLSATMMKTVEDRAPILITRQNGEACVLMSLEEFNALEETAYLLRSPANARRLMDSIDSLKQGQGVERD
LAE

Download         Length: 252 bp

>AT101526 NZ_CP028584:2535713-2535964 [Pseudomonas aeruginosa]
ATGCGCACGATCAGTTACACCGAAGCCCGGCAGAACCTGTCGGCCACCATGATGAAGACCGTGGAGGATCGCGCCCCGAT
CCTGATTACCCGGCAGAACGGCGAAGCCTGCGTGTTGATGTCCCTGGAAGAGTTCAACGCGCTGGAGGAAACGGCTTATC
TGCTGCGCTCCCCGGCCAATGCCCGCCGCCTGATGGATTCGATTGACAGCCTCAAGCAAGGCCAGGGCGTCGAGCGGGAT
TTGGCCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1C7CBF7

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7C600

References