Detailed information of TA system TA101502

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 148162..148415
• Replicon: plasmid pKPC2_020036
• Accession: NZ_CP028582
• Organism: Klebsiella pneumoniae strain WCHKP36

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: CEA75_RS01390
• Protein ID: WP_001312851.1
• Coordinates: 148266..148415 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	148162..148221 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEA75_RS01345	143822..143887	-	66	Protein_206	hypothetical protein	-
CEA75_RS01350	143940..144644	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
CEA75_RS01355	144708..144869	+	162	Protein_208	DNA helicase	-
CEA75_RS01360	144889..145635	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
CEA75_RS01365	145690..146250	+	561	WP_000139328.1	fertility inhibition protein FinO	-
CEA75_RS01370	146382..146582	+	201	WP_015059022.1	hypothetical protein	-
CEA75_RS01375	146968..147567	+	600	WP_032083981.1	hypothetical protein	-
CEA75_RS01380	147731..147961	+	231	WP_001736714.1	hypothetical protein	-
CEA75_RS01385	147984..148211	-	228	Protein_214	hypothetical protein	-
-	148162..148221	-	60	NuclAT_0	-	Antitoxin
-	148162..148221	-	60	NuclAT_0	-	Antitoxin
-	148162..148221	-	60	NuclAT_0	-	Antitoxin
-	148162..148221	-	60	NuclAT_0	-	Antitoxin
CEA75_RS01390	148266..148415	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
CEA75_RS01395	148699..148947	+	249	WP_000083837.1	replication regulatory protein RepA	-
CEA75_RS01400	148948..149157	-	210	WP_064765370.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / catA2 / blaKPC-2	-	1..149258	149258

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T101502 WP_001312851.1 NZ_CP028582:148266-148415 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T101502 NZ_CP028582:148266-148415 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT101502 NZ_CP028582:c148221-148162 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin