Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3793206..3793747 Replicon chromosome
Accession NZ_CP028578
Organism Escherichia coli strain WCHEC005784

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag DBQ99_RS20960 Protein ID WP_000615983.1
Coordinates 3793469..3793747 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag DBQ99_RS20955 Protein ID WP_000729703.1
Coordinates 3793206..3793466 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBQ99_RS20935 3788881..3789666 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
DBQ99_RS20940 3789638..3791350 + 1713 Protein_3615 flagellar biosynthesis protein FlhA -
DBQ99_RS20945 3791574..3792071 - 498 WP_060581685.1 REP-associated tyrosine transposase RayT -
DBQ99_RS20950 3792247..3792996 - 750 WP_000056849.1 C40 family peptidase -
DBQ99_RS20955 3793206..3793466 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
DBQ99_RS20960 3793469..3793747 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
DBQ99_RS20965 3793903..3794643 + 741 WP_001225679.1 murein L,D-transpeptidase -
DBQ99_RS20970 3794614..3795381 - 768 WP_000333380.1 class II glutamine amidotransferase -
DBQ99_RS20975 3795587..3796165 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA / rhs/PAAR / vgrG/tssI 3700562..3808716 108154
- inside Prophage - gmhA/lpcA / rhs/PAAR / vgrG/tssI 3720875..3808716 87841


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T101496 WP_000615983.1 NZ_CP028578:3793469-3793747 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T101496 NZ_CP028578:3793469-3793747 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT101496 WP_000729703.1 NZ_CP028578:3793206-3793466 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT101496 NZ_CP028578:3793206-3793466 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References