Detailed information of TA system TA101495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3787009..3787703
• Replicon: chromosome
• Accession: NZ_CP028578
• Organism: Escherichia coli strain WCHEC005784

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: DBQ99_RS20920
• Protein ID: WP_001263489.1
• Coordinates: 3787009..3787407 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: DBQ99_RS20925
• Protein ID: WP_000554758.1
• Coordinates: 3787410..3787703 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3782597..3782677	-	81	NuclAT_12	-	-
-	3782597..3782677	-	81	NuclAT_12	-	-
-	3782597..3782677	-	81	NuclAT_12	-	-
-	3782597..3782677	-	81	NuclAT_12	-	-
DBQ99_RS20890	3783273..3783731	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
DBQ99_RS20895	3783992..3785449	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
DBQ99_RS20900	3785506..3786027	-	522	Protein_3608	peptide chain release factor H	-
DBQ99_RS20905	3786023..3786229	-	207	Protein_3609	RtcB family protein	-
DBQ99_RS20915	3786547..3786999	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
DBQ99_RS20920	3787009..3787407	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DBQ99_RS20925	3787410..3787703	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DBQ99_RS20930	3787755..3788810	-	1056	WP_001226164.1	DNA polymerase IV	-
DBQ99_RS20935	3788881..3789666	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
DBQ99_RS20940	3789638..3791350	+	1713	Protein_3615	flagellar biosynthesis protein FlhA	-
DBQ99_RS20945	3791574..3792071	-	498	WP_060581685.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA / rhs/PAAR / vgrG/tssI	3700562..3808716	108154
	-	inside	Prophage	-	gmhA/lpcA / rhs/PAAR / vgrG/tssI	3720875..3808716	87841

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T101495 WP_001263489.1 NZ_CP028578:c3787407-3787009 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T101495 NZ_CP028578:c3787407-3787009 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT101495 WP_000554758.1 NZ_CP028578:c3787703-3787410 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT101495 NZ_CP028578:c3787703-3787410 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3